NOS mRNA in the paraventricular nucleus of young and old rats after immobilization stress.
NOS mRNA in the paraventricular nucleus of young and old rats after immobilization stress.
复制标题
固定应激后年轻和年老大鼠室旁核中 NOS mRNA 的变化。
作者：
L. Calzà;L. Giardino;S. Ceccatelli
一氧化氮合成（NOS）酶已经定位于不同的神经群，包括下丘脑室旁核（PVN）。采用原位杂交技术，我们研究了固定应激2 h后幼龄和老年大鼠PVN中NOS mRNA的可能变化。应激后，老龄大鼠PVN内NOS mRNA增加3倍，而幼龄大鼠在应激结束后3小时处死时，NOS mRNA的增加达到显著水平。目前的研究结果表明，一氧化氮可能参与了PVN的应激反应。年轻大鼠和老年大鼠NOS mRNA的差异可能与年龄依赖性应激刺激反应的差异有关。
Nitric oxide synthesizing (NOS) enzyme has been localized in distinct neural populations, including the hypothalamic paraventricular nucleus (PVN). Using in situ hybridization, we have investigated possible changes in NOS mRNA in the PVN of young and old rats after 2 h of immobilization stress. In old rats a 3-fold increase in NOS mRNA was observed in the PVN immediately after the stressor, whereas in young rats the increase reached significance when animals were killed 3 h after the end of the stress procedure. The present results suggest that nitric oxide in the PVN may be involved in the stress response. The differences observed in NOS mRNA between young and old rats could be related to an age-dependent different responsiveness to stressful stimuli.