In vivo gene delivery and stable transduction of nondividing cells by a lentiviral vector
In vivo gene delivery and stable transduction of nondividing cells by a lentiviral vector
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DOI：
10.1126/science.272.5259.263
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发表时间：
1996-04-12
期刊：
影响因子：
56.9
通讯作者：
Trono, D
中科院分区：
文献类型：
--
作者：
Naldini, L;Blomer, U;Trono, D
研究人员开发了一种基于人类免疫缺陷病毒（HIV）的逆转录病毒载体系统，与基于小鼠白血病病毒的对应系统不同，该系统将异源序列转导到HeLa细胞和细胞周期被阻断的大鼠成纤维细胞中，以及人原代巨噬细胞中。此外，HIV载体可以介导稳定的体内基因转移到终末分化神经元中。基于hiv的病毒载体在体内将基因传递到非分裂细胞的能力可以增加逆转录病毒载体在人类基因治疗中的适用性。
A retroviral vector system based on the human immunodeficiency virus (HIV) was developed that, in contrast to a murine leukemia virus-based counterpart, transduced heterologous sequences into HeLa cells and rat fibroblasts blocked in the cell cycle, as well as into human primary macrophages. Additionally, the HIV vector could mediate stable in vivo gene transfer into terminally differentiated neurons. The ability of HIV-based viral vectors to deliver genes in vivo into nondividing cells could increase the applicability of retroviral vectors in human gene therapy.