The MTT and Crystal Violet Assays: Potential Confounders in Nanoparticle Toxicity Testing
The MTT and Crystal Violet Assays: Potential Confounders in Nanoparticle Toxicity Testing
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DOI：
10.1177/1091581816648906
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发表时间：
2016-07-01
影响因子：
2.2
通讯作者：
Sanderson, B. J. S.
中科院分区：
文献类型：
--
作者：
Almutary, A.;Sanderson, B. J. S.
纳米颗粒（NPs）对人类、动物和环境的毒理学效应在很大程度上是未知的。NP细胞毒性的评估取决于试验系统的选择。由于NP的光学活性和吸收值，它们可以影响经典的细胞毒性测定。在3-（4，5-二甲基噻唑-2-基）-2，5-二苯基四唑溴化物（MTT）和结晶紫测定中掺入8种NP，并用HaCaT人皮肤细胞进行测试。MTT测定标准曲线光密度（OD）测量值因三硅烷醇苯基和三硅烷醇异辛基多面体低聚倍半硅氧烷颗粒的存在而改变。结晶紫标准曲线OD测量值被金纳米颗粒显著偏移，但它们不影响MTT测定。炭黑在MTT和结晶紫测定中降低OD，并且定位于细胞质中。这些发现强烈表明，需要仔细选择体外活力系统，以避免有缺陷的测量纳米颗粒的毒性。
The toxicological effects of nanoparticles (NPs) on humans, animals, and environment are largely unknown. Assessment of NPs cytotoxicity depends on the choice of the test system. Due to NPs optical activity and absorption values, they can influence the classical cytotoxicity assay. Eight NPs were spiked in the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and crystal violet assays and tested with HaCaT human skin cells. The MTT assay standard curve optical density (OD) measurements were altered by the presence of trisilanol phenyl and trisilanol isooctyl polyhedral oligomeric silsesquioxane particles. The crystal violet standard curve OD measurements were significantly shifted by gold NPs, but they did not affect the MTT assay. Carbon black decreased ODs in the MTT and crystal violet assays and was localized in the cell cytoplasm. These findings strongly indicate that a careful choice of in vitro viability systems is required to avoid flawed measurement of NPs toxicity.