Handling of gametes for in vitro insemination in birds.
Handling of gametes for in vitro insemination in birds.
复制标题
鸟类体外授精配子的处理。
DOI：
10.1007/978-1-4939-7216-6_16
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发表时间：
2017
影响因子：
--
通讯作者：
Tomohiro Sasanami
中科院分区：
文献类型：
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作者：
Shusei Mizushima;Mei Matsuzaki;Tomohiro Sasanami
鸟类配子的生物学特性（例如，巨大的卵子和多精子受精）不允许在体外直接观察精子与卵子的相互作用，但最近的研究进展使在体外操纵配子成为可能。在这里，我们描述的技术处理配子所需的体外受精试验。此外，我们还介绍了精子-卵黄周膜测定，胞浆内精子注射和卵外培养的程序。
A characteristic biological property of avian gamete (e.g., extremely large egg and polyspermic fertilization) does not allow the direct observation of sperm-egg interactions in vitro, but recent research advances make it possible to manipulate the gamete in vitro. Here, we describe the techniques for the handling of gametes required for in vitro fertilization assay. In addition, we also introduce the procedures for sperm-perivitelline membrane assay, intracytoplasmic sperm injection, and ex ovo culture.