Strategies for induction of protective immunity to bovine herpesvirus-1 in newborn calves with maternal antibodies
Strategies for induction of protective immunity to bovine herpesvirus-1 in newborn calves with maternal antibodies
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DOI：
10.1016/j.vaccine.2008.02.018
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发表时间：
2008-06-13
期刊：
影响因子：
5.5
通讯作者：
Babiuk, L. A.
中科院分区：
文献类型：
--
作者：
den Hurk, S. van Drunen Littel-van;Snider, M.;Babiuk, L. A.
本研究的目的是评估Th1促进新生儿接种牛疱疹病毒-1 （BHV-1）疫苗的策略。一种编码分泌的糖蛋白D （tgD）的截断型质粒和CpG寡脱氧核苷酸（ODN）配制的tgD蛋白可以有效地启动新生羔羊的免疫系统，而没有CpG ODN的tgD蛋白则效果较差。此外，与DNA疫苗或蛋白质/CpG启动物DNA增强相比，外源DNA启动物蛋白/CpG启动物DNA增强诱导的免疫反应更强、更平衡。研究人员比较了三种方法诱导具有bhv -1特异性母源抗体的新生小牛的保护性免疫。与安慰剂组相比，DNA疫苗诱导的保护作用微乎其微，而DNA启动蛋白的增强导致体温反应降低、体重减轻和病毒脱落。用蛋白质/CpG配方免疫的小牛对BHV-1的攻击产生了接近完全的保护，因为这些动物体重减轻很少，温度只有轻微升高，几乎没有病毒脱落。(C) 2008 Etsevier Ltd版权所有。
The objective of this study was to evaluate Th1 promoting strategies for vaccination of neonates against bovine herpesvirus-1 (BHV-1). A plasmid encoding a secreted truncated version of glycoprotein D (tgD) and tgD protein formulated with CpG oligodeoxynucleotide (ODN) effectively primed the immune system of newborn lambs, whereas without CpG ODN the tgD protein was less effective. Furthermore, a heterologous DNA prime-protein/CpG boost induced stronger and more balanced immune responses than either the DNA vaccine or a protein/CpG prime-DNA boost. Three of these strategies were compared as an approach to induce protective immunity in newborn calves with BHV-1-specific maternal antibodies. Whereas the DNA vaccine induced minimal protection, the DNA prime-protein boost resulted in reduced temperature response, weight loss and virus shedding in comparison to the placebo group. Close to complete protection against BHV-1 challenge was elicited in the calves immunized with the protein/CpG formulation, as these animals lost very little weight, had only slightly elevated temperatures and shed almost no virus. (C) 2008 Etsevier Ltd. All rights reserved.