STUDIES IN DIABETIC MUTANT MOUSE .1. LIGHT MICROSCOPY AND RADIOAUTOGRAPHY OF PANCREATIC ISLETS
STUDIES IN DIABETIC MUTANT MOUSE .1. LIGHT MICROSCOPY AND RADIOAUTOGRAPHY OF PANCREATIC ISLETS
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DOI：
10.1007/bf01212231
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发表时间：
1970-01-01
期刊：
影响因子：
8.2
通讯作者：
CHICK, WL
中科院分区：
文献类型：
--
作者：
LIKE, AA;CHICK, WL
在正常小鼠（C 57 BL/KsJ）和糖尿病突变小鼠（C 57 BL/Ks-db/db）中进行光镜和放射自显影研究。正常小鼠的胰岛较小，β细胞颗粒状良好，并且罕见的胰岛细胞掺入胸苷-3H。在高血糖前突变体（血糖< 120 mg/100 ml）中，β细胞也颗粒化良好，但掺入胸苷-3H的频率高于正常小鼠。随着早期高血糖（血糖130-200 mgm/100 ml）和高胰岛素血症的发生，胰岛大小正常，但β细胞部分脱颗粒，大量胰岛细胞被胸苷-3H标记。在高血糖（血糖< 200 mg/100 ml）的情况下，血清IRI显著增加，β细胞脱粒明显，标记的胰岛细胞数量众多，胰岛明显增大。该综合征的终末期的特征是β细胞数量减少和小导管结构独特的增殖。标记的导管细胞众多，标记的内分泌胰岛细胞罕见，动物在相对胰岛素不足的状态下死亡。
Light microscopic and radioautographic studies were performed in normal (C 57 BL/KsJ) and in diabetic mutant (C 57 BL/Ks-db/db) mice following thymidine-3H administration. The pancreatic islets of normal mice were small, beta cells well granulated and a rare islet cell incorporated thymidine-3H. In prehyperglycemic mutants (blood glucose < 120 mg/100 ml) beta cells were also well granulated but incorporated thymidine-3H with greater frequency than observed in normal mice. With the onset of early hyperglycemia (blood glucose 130–200 mgm/100ml) and hyperinsulinemia, the islets were normal in size, however beta cells were partially degranulated and numerous islet cells were labeled with thymidine-3H. With established hyperglycemia (blood glucose < 200 mg/100 ml) serum IRI was greatly increased, beta cell degranulation pronounced, labeled islet cells numerous, and islet enlargement noteworthy. The terminal phase of the syndrome was characterized by a decrease in the number of beta cells and a unique proliferation of small ductal structures. Labeled ductal cells were numerous, labeled endocrine islet cells infrequent and the animals succumbed in a state of relative insulin insufficiency.