SUPEROXIDE RADICAL GENERATION, NADPH OXIDASE ACTIVITY, AND CYTOCHROME-P-450 CONTENT OF RAT-LIVER MICROSOMAL FRACTIONS IN AN EXPERIMENTAL HYPERTHYROID STATE - RELATION TO LIPID-PEROXIDATION
SUPEROXIDE RADICAL GENERATION, NADPH OXIDASE ACTIVITY, AND CYTOCHROME-P-450 CONTENT OF RAT-LIVER MICROSOMAL FRACTIONS IN AN EXPERIMENTAL HYPERTHYROID STATE - RELATION TO LIPID-PEROXIDATION
复制标题
DOI：
10.1210/endo-117-2-496
复制
发表时间：
1985-01-01
期刊：
影响因子：
4.8
通讯作者：
VIDELA, LA
中科院分区：
文献类型：
--
作者：
FERNANDEZ, V;BARRIENTOS, X;VIDELA, LA
在连续1、2、3和7天给予0.1 mg T3/kg [体重]的大鼠中研究了甲状腺激素处理对与NADPH依赖性电子转移反应相关的肝微粒体功能的影响。该处理导致O2-的速率增加。在表现出呼吸增加的肝脏中，伴随着NADPH氧化酶活性的增强和细胞色素P-450含量的降低，通过微粒体组分产生。随后的研究表明，微粒体组分和肝匀浆中丙二醛水平升高，以及后者系统中的荧光反应增强。显然，T3 [三碘甲腺原氨酸]对肝组织的产热作用伴随着对微粒体功能的刺激，涉及O的单价还原。这种细胞反应可能导致更大的脂质过氧化率和细胞色素P-450损失作为甲状腺激素作用的次要事件。
The effect of thyroid hormone treatment on hepatic microsomal functions related to NADPH-dependent electron transfer reactions was studied in rats given 0.1 mg T3/kg [body wt] for 1, 2, 3 and 7 consecutive days. This treatment resulted in increased rates of O2- .cntdot. generation by microsomal fractions, concomitantly with an enhancement in NADPH oxidase activity and decreased cytochrome P-450 content, in livers exhibiting increased respiration. Subsequent studies showed elevated levels of malondialdehyde in microsomal fractions and liver homogenates, as well as augmented chemiluminescent response in the latter system. Apparently, the calorigenic effect of T3 [triiodothyronine] on the liver tissue is accompanied by a stimulation of microsomal functions involving univalent reduction of O. This cellular response might lead to a greater lipid peroxidative rate and cytochrome P-450 loss as secondary events of thyroid hormone action.