IDENTIFICATION AND REGULATION OF A GENE REQUIRED FOR CELL-FUSION DURING MATING OF THE YEAST SACCHAROMYCES-CEREVISIAE
IDENTIFICATION AND REGULATION OF A GENE REQUIRED FOR CELL-FUSION DURING MATING OF THE YEAST SACCHAROMYCES-CEREVISIAE
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DOI：
10.1128/mcb.7.8.2680
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发表时间：
1987-08-01
影响因子：
5.3
通讯作者：
SPRAGUE, GF
中科院分区：
文献类型：
--
作者：
MCCAFFREY, G;CLAY, FJ;SPRAGUE, GF
我们已经设计了一种酵母基因的筛选，这些基因的表达受到细胞类型或交配信息素的影响。从这个筛选中，我们确定了一个基因，FUS1，它的表达模式揭示了有趣的调控策略，其产物是交配过程中高效细胞融合所必需的。FUS1的转录只在α和α中发生。单元格，而不是a/.pha。细胞，在那里它被A1.cntdot抑制。α2，在a/α中唯一存在的一种调节活动。细胞。转录结果显示，FUS1对STE4、STE5、STE7、STE11和STE12 5个STE基因的产物有绝对需求。因为激活剂STE4、STE5和STE12本身被A1·cntdot抑制。α2，未能在a/α中表达FUS1。细胞可能是一系列调控活动的结果；抑制A1.cntdot的激活物。α2进而阻止FUS1的转录。除了细胞类型对FUS1的调控外，当a或α时，该基因座的转录增加了10倍或更多。细胞暴露在相反的交配信息素中。为了研究FUS1蛋白的功能，我们创建了FUS1缺失突变体。在FUS1。时代。FUS1交配时，交配对的细胞紧密结合，形成合子。然而，受精卵是异常的。在接合桥内，它们含有阻止细胞器核融合和混合的隔板。FUS1蛋白的预测序列(由FUS1DNA序列推导而来)和FUS1-β-半乳糖苷酶杂合蛋白的亚细胞分级研究表明，FUS1是一种膜蛋白或分泌型蛋白。因此，FUS1可能位于细胞内的某个位置，在那里它可以催化细胞壁或质膜融合。
We have devised a screen for genes from the yeast Saccharomyces cerevisiae whose expression is affected by cell type or by the mating pheromones. From this screen we identified a gene, FUS1, whose pattern of expression revealed interesting regulatory strategies and whose product was required for efficient cell fusion during mating. Transcription of FUS1 occurred only in a and .alpha. cells, not in a/.alpha. cells, where it was repressed by a1 .cntdot. .alpha.2, a regulatory activity present uniquely in a/.alpha. cells. Transcription of FUS1 showed an absolute requirement for the products of five STE genes, STE4, STE5, STE7, STE11, and STE12. Since the activators STE4, STE5, and STE12 are themselves repressed by a1 .cntdot. .alpha.2, the failure to express FUS1 in a/.alpha. cells is probably the result of a cascade of regulatory activities; repression of the activators of a1 .cntdot. .alpha.2 in turn precludes transcription of FUS1. In addition to regulation of FUS1 by cell type, transcription from the locus increased 10-fold or more when a or .alpha. cells were exposed to the opposing mating pheromone. To investigate the function of the Fus1 protein, we created fus1 null mutants. In fus1 .times. fus1 matings, the cells of a mating pair adhered tightly and appeared to form zygotes. However, the zygotes were abnormal. Within the conjugation bridge they contained a partition that prevented nuclear fusion and mixing of organelles. The predicted sequence of the Fus1 protein (deduced from the FUS1 DNA sequence) and subcellular fractionation studies with Fus1-.beta.-galactosidase hybrid proteins suggest that Fus1 is a membrane or secreted protein. Thus, Fus1 may be located at a position within the cell where it is poised to catalyze cell wall or plasm membrane fusion.