Conjugated Polymer Based Nanoparticles as Dual-Modal Probes for Targeted In Vivo Fluorescence and Magnetic Resonance Imaging
Conjugated Polymer Based Nanoparticles as Dual-Modal Probes for Targeted In Vivo Fluorescence and Magnetic Resonance Imaging
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基于共轭聚合物的纳米粒子作为双模态探针用于体内靶向荧光和磁共振成像
DOI：
10.1002/adfm.201102234
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发表时间：
2012-08-07
影响因子：
19
通讯作者：
Liu, Bin
中科院分区：
文献类型：
--
作者：
Li, Kai;Ding, Dan;Liu, Bin
通过将远红/近红外(FR/NIR)发射共轭聚合物(PFVBT)和脂质包裹的氧化铁(IO)共包裹在聚乳酸-乙醇酸-聚乙二醇叶酸酯(PLGA-PEGFOL)和PLGA的混合物中，开发了一种简便的方法来合成带有表面叶酸的双模式荧光磁性纳米颗粒(NPs)。得到的纳米粒子具有超顺磁性和高荧光，这表明包裹在IOS上的脂类可以有效地将共轭聚合物从IOS中分离出来，从而最大限度地减少荧光猝灭。激光光散射结果表明，这些纳米粒子呈球形，在水中的平均直径约为180 nm。体外研究表明，这些双峰纳米粒子可以作为一种有效的荧光探针，在没有明显细胞毒性的情况下实现对MCF-7乳腺癌细胞的靶向成像。体内荧光和磁共振成像结果表明，NPs能够优先在肿瘤组织中积聚，从而允许双模式检测活体中的肿瘤。这表明基于共轭聚合物的双模式纳米探针在未来的体外和体内应用中具有广泛的潜力。
A facile strategy is developed to synthesize dual-modal fluorescent-magnetic nanoparticles (NPs) with surface folic acid by co-encapsulation of a far-red/near-infrared (FR/NIR)-emissive conjugated polymer (PFVBT) and lipid-coated iron oxides (IOs) into a mixture of poly(lactic-co-glycolic-acid)-poly(ethylene glycol)-folate (PLGA-PEG-FOL) and PLGA. The obtained NPs exhibit superparamagnetic properties and high fluorescence, which indicates that the lipid coated on IOs is effective at separating the conjugated polymer from IOs to minimize fluorescence quenching. These NPs are spherical in shape with an average diameter of approximate to 180 nm in water, as determined by laser light scattering. In vitro studies reveal that these dual-modal NPs can serve as an effective fluorescent probe to achieve targeted imaging of MCF-7 breast cancer cells without obvious cytotoxicity. In vivo fluorescence and magnetic resonance imaging results suggest that the NPs are able to preferentially accumulate in tumor tissues to allow dual-modal detection of tumors in a living body. This demonstrates the potential of conjugated polymer based dual-modal nanoprobes for versatile in vitro and in vivo applications in future.