Enhanced in vivo responses of osteoblasts in electrostatically activated zones by hydroxyapatite electrets
Enhanced in vivo responses of osteoblasts in electrostatically activated zones by hydroxyapatite electrets
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DOI：
10.1007/s10856-008-3546-7
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发表时间：
2009-01-01
影响因子：
3.7
通讯作者：
Yamashita, Kimihiro
中科院分区：
文献类型：
--
作者：
Nakamura, Satoshi;Kobayashi, Takayuki;Yamashita, Kimihiro
成熟的成骨细胞被证明是位于诱导的大的表面电荷的电极化羟基磷灰石（HA）陶瓷上加速骨形成的电荷极性无关，但空间细胞分布模式是不同的。将平均电荷为3.9A μ Ccm（-2）的极化HA陶瓷板植入犬股骨宽间距缺损中3天和7天。骨钙素、骨桥蛋白免疫组化法鉴定成骨细胞。骨钙素和骨桥蛋白的表达在整个植入的HA板和切割的皮质骨表面之间的间隙中被检测到，特别是在切割的皮质骨表面和类骨质的附近，无论感应电荷的极性如何。此外，直接结合到带负电荷的HA表面的新形成的骨组织衬有成骨细胞层。植入后7天，发育良好的成骨细胞的存在表明，HA陶瓷的静电力调节了极化HA表面的生物界面区的电场。
Matured osteoblasts were proved to be located in the bone formation accelerated by induced large surface charges on the electrically polarized hydroxyapatite (HA) ceramics regardless of the charge polarities, whereas the spatial cell distribution patterns were different. Polarized HA ceramic plates with an average electric charge of 3.9 A mu Ccm(-2) were implanted in widely spaced defects of canine femora for 3 and 7 days. The osteoblasts were identified by immunochemical detections of osteocalcin and osteopontin. Expressions of osteocalcin and osteopontin were detected throughout the gaps between the implanted HA plates and the cut cortical bone surfaces, especially in the vicinities of the cut cortical bone surfaces and the osteoids regardless of the polarity of the induced charges. Additionally, the newly formed bone tissue that directly bonded to the negatively charged HA surfaces was lined by an osteoblast layer. As soon as 7 days after the implantation, the presence of well-developed osteoblasts suggested that the electrostatic force of the HA ceramics had conditioned the field in the biointerface zone of the polarized HA surfaces.