Effect of nef alleles on replication of human immunodeficiency virus type 1.
Effect of nef alleles on replication of human immunodeficiency virus type 1.
复制标题
nef等位基因对人类免疫缺陷病毒1型复制的影响。
DOI：
10.1006/viro.1993.1230
复制
发表时间：
1993
期刊：
影响因子：
3.7
通讯作者：
Haseltine,WA
中科院分区：
文献类型：
--
作者：
Zazopoulos,E;Haseltine,WA
本文研究了人类免疫缺陷病毒Ⅰ型（HIV-1）nef的多个等位基因对病毒复制的影响，所用的nef等位基因包括一些来自组织培养传代的病毒分离株，以及另一些来自感染患者组织的病毒DNA直接克隆获得的nef等位基因。nefon病毒复制的影响是在HXB 2前病毒衍生物的背景下进行评价的，该前病毒衍生物先前显示为在CD 4+人T细胞系和原代外周血单核细胞中快速生长所需。实验结果表明，在这种遗传背景下，所有研究的携带nef基因的病毒都能表达稳定的Nef蛋白，它们的复制速度比其他同基因的nef缺陷型病毒更快。两个来自初生组织的等位基因产生不稳定的蛋白质。这些研究表明，自然发生的nefalleles可以增加病毒在原代外周血单核细胞和CD 4 +T细胞系中的复制率。结果还表明，功能变异存在于自然发生的nef等位基因。
The effect of multiple alleles ofnefof the human immunodeficieney virus type 1 (HIV-1) on virus replication was examined.Nefalleles used include some derived from isolates of virus passaged in tissue culture as well as others obtained by direct cloning of viral DNA from tissues of infected patients. The effect ofnefon virus replication was evaluated in the context of a derivative of the HXB2 provirus shown previously to requireneffor rapid growth in CD4+human T cell lines and in primary peripheral blood mononuclear cells. The results of the experiments show that in this genetic context all of the studied viruses carryingnefalleles that express stable Nef proteins replicate more rapidly than do their, otherwise isogenic,nef-defective counterparts. Two of thenefalleles derived from primary tissues produce unstable proteins. These studies demonstrate that naturally occurringnefalleles can increase the rate of virus replication in both primary peripheral blood mononuclear cells and in a CD4+T cell line. The results also demonstrate that functional variation exists among naturally occurring nef alleles.