THE COENRICHMENT OF STEM-CELLS, PROTHYMOCYTES, AND STROMAL ELEMENTS WITH ECOTROPIC RETROVIRUS-PRODUCING CELLS FROM THE BONE-MARROW OF LEUKEMIA-PRONE AKR MICE
THE COENRICHMENT OF STEM-CELLS, PROTHYMOCYTES, AND STROMAL ELEMENTS WITH ECOTROPIC RETROVIRUS-PRODUCING CELLS FROM THE BONE-MARROW OF LEUKEMIA-PRONE AKR MICE
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DOI：
10.1016/0042-6822(88)90475-8
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发表时间：
1988-02-01
期刊：
影响因子：
3.7
通讯作者：
WEINHOLD, KJ
中科院分区：
文献类型：
--
作者：
BUCKHEIT, RW;KURTZBERG, J;WEINHOLD, KJ
亲嗜性病毒产生细胞在骨髓中的白血病倾向的AKR株的小鼠显着富集的不连续密度梯度的Percoll上的分馏，并发现在低密度的细胞群主要由中到大的原始细胞。还发现高亲嗜性病毒产生的低密度骨髓细胞群体中多能干细胞、前胸腺细胞和基质成分显著富集。在AKR小鼠成功的白血病抑制免疫治疗的窗口所定义的时间段内，仅在这部分骨髓细胞中检测到产病毒细胞，这表明在这部分中共富集的细胞的功能类别既是抗病毒免疫治疗的潜在靶点，也是感染性嗜亲性病毒在脾脏和胸腺中形成苗的原因。
Ecotropic virus-producing cells in the bone marrow of the leukemia-prone AKR strain of mice were significantly enriched by fractionation on discontinuous density gradients of Percoll and were found in a low-density population of cells comprised predominantly of medium to large blast cells. The high ecotropic virus-producing low-density bone marrow cell population was also found to be significantly enriched in pluripotent stem cells, prothymocytes, and stromal elements. During the period of time defined by a window for successful leukemosuppressive immunotherapy of AKR mice, virus-producing cells were exclusively detected in this fraction of bone marrow cells, implicating the functional classes of cells coenriched in this fraction as both potential targets of anti-viral immunotherapy and responsible for the seedling of the spleen and thymus with infectious ecotropic virus.