Increased adhesion of erythrocytes to components of the extracellular matrix: Isolation and characterization of a red blood cell lipid that binds thrombospondin and laminin
Increased adhesion of erythrocytes to components of the extracellular matrix: Isolation and characterization of a red blood cell lipid that binds thrombospondin and laminin
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DOI：
10.1182/blood.v87.11.4879.bloodjournal87114879
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发表时间：
1996-06-01
期刊：
影响因子：
20.3
通讯作者：
Scott, JP
中科院分区：
文献类型：
--
作者：
Hillery, CA;Du, MC;Scott, JP
红细胞（RBC）对血管内皮的粘附在一些病理条件下增加，包括镰状细胞病和疟疾。然而，红细胞与内皮下基质成分的相互作用并没有很好地表征。在1达因/厘米(2)的体外流速条件下，洗涤后的红细胞与纯化的粘附分子血栓反应蛋白（TSP）和层粘连蛋白结合。镰状红细胞在所有测试红细胞中具有最大的粘附性。高分子量（MW）硫酸葡聚糖和硫酸软骨素A可抑制镰状红细胞对固定TSP的粘附，而其他具有相似结构和电荷密度的阴离子多糖则无抑制作用。这些数据与RBC黏附分子为硫酸化糖脂一致。因此。从正常红细胞和镰状红细胞中分离并鉴定了tsp结合脂质。采用碱性甲醇解、阴离子交换层析、制备薄层析等方法纯化tsp结合脂质。采用特殊的覆盖tsp结合试验，在TLC上确定了均匀带。TSP与纯化脂质的结合在碱和神经氨酸酶处理下是稳定的，在酸处理下是不稳定的，并且被高分子量的葡聚糖硫酸盐所抑制，类似于在流动条件下完整红细胞与固定TSP的结合。此外，可溶性层粘连蛋白与纯化的红细胞脂结合。这种从镰状红细胞和正常红细胞膜中分离出来的酸性TSP-和层粘连蛋白结合脂质可能有助于红细胞与内皮下基质的相互作用，从而参与血管闭塞性疾病的发病机制。(C) 1996年由美国血液病学会出版。
Red blood cell (RBC) adhesion to the vascular endothelium is increased in several pathologic conditions, including sickle cell disease and malaria. However, RBC interactions with components of the subendothelial matrix are not well-characterized. Under in vitro flow conditions of 1 dyne/cm(2), washed RBCs bound to the purified adhesive molecules thrombospondin (TSP) and laminin. Sickle RBCs had the greatest adhesion of all tested RBCs. The adhesion of sickle RBCs to immobilized TSP was inhibited by the anionic polysaccharides high molecular weight (MW) dextran sulfate and chondroitin sulfate A, but not other anionic polysaccharides of similar structure and/or charge density. These data were consistent with the RBC adhesive molecule being a sulfated glycolipid. Therefore. TSP-binding lipids from normal and sickle RBCs were isolated and characterized. The TSP-binding lipid was purified by alkaline methanolysis, anion exchange chromatography and preparative thin layer chromatography (TLC). A homogeneous band on TLC was identified using a specific overlay TSP-binding assay. TSP binding to the purified lipid was stable to base and neuraminidase treatment, labile to acid treatment, and was inhibited by high MW dextran sulfate, similar to that seen with intact RBCs binding to immobilized TSP under conditions of flow. In addition, soluble laminin bound to the purified RBC lipid. This acidic TSP- and laminin-binding lipid(s) isolated from both sickle and normal RBC membranes may contribute to erythrocyte interactions with the subendothelial matrix, thereby participating in the pathogenesis of vaso-occlusive diseases. (C) 1996 by The American Society of Hematology.