Distinct tissue distribution and cellular localization of two messenger ribonucleic acids encoding different subtypes of rat endothelin receptors.
Distinct tissue distribution and cellular localization of two messenger ribonucleic acids encoding different subtypes of rat endothelin receptors.
复制标题
编码大鼠内皮素受体不同亚型的两种信使核糖核酸的不同组织分布和细胞定位。
作者：
S. Hori;Y. Komatsu;R. Shigemoto;N. Mizuno;S. Nakanishi
内皮素（ETs）是一种非常有效的血管收缩肽，在血管和非血管组织中具有多种功能。本研究涉及编码两种不同ET受体亚型（ETA和ETB）的大鼠mrna的组织分布和细胞定位。通过与牛ETA cDNA杂交，从大鼠肺cDNA文库中分离出46个cDNA克隆。这些cDNA克隆的特征表明它们代表ETA或ETB cDNA。原位杂交和印迹杂交分析显示，大鼠ETA mRNA主要表达于多种组织的血管平滑肌细胞、支气管平滑肌细胞、心肌和垂体。血管平滑肌细胞中没有明显的ETB mRNA表达，因此，ETA在et诱导的血管收缩中起主要作用。ETB mRNA更广泛地分布于许多组织的各种细胞类型中。在整个脑区的神经胶质细胞、脉络膜丛上皮细胞、心室室管膜细胞、心肌、肾小球内皮细胞和Henle’s袢薄段上皮细胞中可见其显著表达。我们的研究表明，大鼠ET受体的两种亚型mrna在大脑和外周组织中都表现出细胞类型的特化表达模式。
Endothelins (ETs) are very potent vasoconstrictive peptides and have diverse functions in both vascular and nonvascular tissues. This investigation concerns the tissue distribution and cellular localization of rat mRNAs encoding two different subtypes of ET receptors (ETA and ETB). We isolated 46 cDNA clones from a rat lung cDNA library by hybridization with the bovine ETA cDNA. The characterization of these cDNA clones indicated that they represent either the ETA or ETB cDNA. In situ and blot hybridization analyses revealed that the rat ETA mRNA is predominantly expressed in vascular smooth muscle cells of a variety of tissues, bronchial smooth muscle cells, myocardium, and the pituitary gland. There is no significant expression of ETB mRNA in vascular smooth muscle cells, and ETA, thus, plays a primary role in ET-induced vascular contraction. ETB mRNA is more widely distributed in various cell types of many tissues. Its prominent expression is seen in glial cells throughout the brain regions, epithelial cells of the choroid plexus, ependymal cells lining the ventricle, myocardium, endothelial cells of glomeruli, and epithelial cells of the thin segments of Henle's loops. Our investigation demonstrates that the mRNAs for the two subtypes of rat ET receptors show specialized expression patterns of cell types in both brain and peripheral tissues.
细胞表面内皮素受体的异质性。
DOI：
--
发表时间：
1990
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Martin,ER;Brenner,BM;Ballermann,BJ
通讯作者：
Ballermann,BJ
DOI：
10.1073/pnas.87.6.2359
发表时间：
1990-03-01
影响因子：
11.1
作者：
MACCUMBER, MW;ROSS, CA;SNYDER, SH
通讯作者：
SNYDER, SH