Specificity of a polymerase chain reaction assay of a target sequence on the 31-kilodalton Brucella antigen DNA used to diagnose human brucellosis
Specificity of a polymerase chain reaction assay of a target sequence on the 31-kilodalton Brucella antigen DNA used to diagnose human brucellosis
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DOI：
10.1007/pl00011242
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发表时间：
2001-02-01
影响因子：
4.5
通讯作者：
Morata, P
中科院分区：
文献类型：
--
作者：
Casañas, MC;Queipo-Ortuño, MI;Morata, P
本研究的目的是评价聚合酶链反应检测布鲁氏菌DNA的特异性，使用特异性引物扩增编码31 kDa免疫原性流产布鲁氏菌蛋白（BCSP31）的序列的223 bp区域。正确扩增了所有布鲁氏菌菌株（包括型、参考、疫苗和田间菌株）的DNA。除了赭杆菌属之外，在血清学或遗传学上与布鲁氏菌属相关的一组微生物中未检测到其他扩增。这种非常好的特异性程度，以及在以前的临床研究中证明的高产量，证实了这种聚合酶链反应检测可能是一种有用的工具，用于诊断人布鲁氏菌病。
The aim of this study was to evaluate the specificity of a polymerase chain reaction assay for detecting Brucella DNA using primers specific for the amplification of a 223 bp region of the sequence encoding a 31 kDa immunogenic Brucella abortus protein (BCSP31). DNA from all Brucella strains, including type, reference, vaccine and field strains, were correctly amplified. With the exception of Ochro-bactrum spp., no other amplification was detected with a broad panel of microorganisms serologically or phylogenetically related to Brucella spp. This very good degree of specificity, together with its high yield demonstrated in previous clinical studies, confirms that this polymerase chain reaction assay could be a useful tool for the diagnosis of human brucellosis.