Antitumor effects of Scutellariae radix and its components baicalein, baicalin, and wogonin on bladder cancer cell lines
Antitumor effects of Scutellariae radix and its components baicalein, baicalin, and wogonin on bladder cancer cell lines
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DOI：
10.1016/s0090-4295(00)00467-2
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发表时间：
2000-06-01
期刊：
影响因子：
2.1
通讯作者：
Kishimoto, T
中科院分区：
文献类型：
--
作者：
Ikemoto, S;Sugimura, K;Kishimoto, T
目标.目的：研究黄芩及其有效成分黄芩素、黄芩苷和汉黄芩素对人膀胱癌细胞株（KU-1和EJ-1）和小鼠膀胱癌细胞株（MBT-2）的抗肿瘤作用。将膀胱癌细胞与各种浓度的试剂一起孵育。通过3-（4，5-二甲基硫乙醇-2-基）-2，5-二烯基溴化四唑测定法检测对膀胱癌细胞系的抗增殖活性。在体内实验中，小鼠皮下注射MBT-2细胞，并从第11天至第20天以2或10 mg/只的剂量每天一次口服给药黄芪，连续10天。所有药物均呈剂量依赖性地抑制细胞增殖，但黄芩苷表现出最大的抗增殖活性。获得50%抑制所需的黄芩苷浓度对于KU-1为3.4 μ g/mL，对于EJ-1为4.4 μ g/mL，对于MBT-2为0.93 μ g/mL。对于KU-1和MBT-2，细胞存活率显著降低（P
Objectives. To investigate the antitumor effects of Scutellariae radix and its components baicalein, baicalin, and wogonin on human bladder cancer cell lines (KU-1 and EJ-1) and a murine bladder cancer cell line (MBT-2).Methods. Bladder cancer cells were incubated with various concentrations of the agents. Antiproliferative activity against the bladder cancer cell lines was examined by 3-(4,5-dimethylthianol-2-yl)-2,5-diplenyl tetrazolium bromide assay. In an in vivo experiment, the mice were subcutaneously injected with MBT-2 cells, and Scutellariae radix was orally administered at a dose of 2 or 10 mg per mouse one time daily for 10 days from day 11 to day 20.Results. All the drugs inhibited cell proliferation in a dose-dependent manner, but baicalin exhibited the greatest antiproliferative activity. The concentration of baicalin necessary to obtain 50% inhibition was 3.4 mu g/mL for KU-1, 4.4 mu g/mL for EJ-1, and 0.93 mu g/mL for MBT-2. For KU-1 and MBT-2, the percentage of cell survival significantly decreased (P