Arabidopsis Group IIId ERF proteins positively regulate primary cell wall-type CESA genes
Arabidopsis Group IIId ERF proteins positively regulate primary cell wall-type CESA genes
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DOI：
10.1007/s10265-018-1074-1
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发表时间：
2018-11
影响因子：
2.8
通讯作者：
Laddawan Saelim;Nobuhiro Akiyoshi;T. Tan;Ayumi Ihara;M. Yamaguchi;K. Hirano;M. Matsuoka;T. Demura;M. Ohtani
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作者：
Laddawan Saelim;Nobuhiro Akiyoshi;T. Tan;Ayumi Ihara;M. Yamaguchi;K. Hirano;M. Matsuoka;T. Demura;M. Ohtani
细胞壁决定了植物细胞的形态和对环境的反应。原代细胞壁（PCW）在细胞分裂和扩增过程中产生，决定细胞的形状和体积。经过细胞扩增，特定类型的植物细胞产生木质化的细胞壁，称为次生细胞壁（SCW）。我们对iii族拟南芥AP2/EREBP基因进行了功能分析，即erf34、ERF35、ERF38、andERF39，这些基因是先前被认为与SCW生物合成有关的水稻erf基因的同源物。表达分析表明，这4个基因在幼苗（茎尖分生组织、叶片和侧根原基、毛状体和初生根中心筒）和花（花器官和复瓣的维管组织和雌蕊瓣缘）中与细胞分裂和/或细胞分化有关的区域均有表达。在拟南芥幼苗中，过表达erfgenes可显著上调编码纤维素合成酶催化亚基的pcw型CESAgenes，而不上调scw型CESAgenes。瞬时共表达报告基因分析表明，ERF35、ERF38、anderf39具有转录激活因子活性，ERF34、ERF35、ERF38和ERF39通过AP2/ERF蛋白识别的核心顺式作用元件drectcoreat元件上调pcw型esagene cesa1的启动子活性。总之，我们的研究结果表明，IIIdERFgenes是拟南芥中pcw型ecesagenes的正转录调节因子，并可能参与调节纤维素的生物合成，以响应发育需求和环境刺激。
The cell wall determines morphology and the environmental responses of plant cells. The primary cell wall (PCW) is produced during cell division and expansion, determining the cell shape and volume. After cell expansion, specific types of plant cells produce a lignified wall, known as a secondary cell wall (SCW). We functionally analyzed Group IIId Arabidopsis AP2/EREBP genes, namelyERF34, ERF35, ERF38, andERF39, which are homologs of a riceERFgene previously proposed to be related to SCW biosynthesis. Expression analysis revealed that these four genes are expressed in regions related to cell division and/or cell differentiation in seedlings (i.e., shoot apical meristems, the primordia of leaves and lateral roots, trichomes, and central cylinder of primary roots) and flowers (i.e., vascular tissues of floral organs and replums and/or valve margins of pistils). Overexpression ofERFgenes significantly upregulated PCW-type, but not SCW-type,CESAgenes encoding cellulose synthase catalytic subunits in Arabidopsis seedlings. Transient co-expression reporter analysis indicated thatERF35, ERF38, andERF39possess transcriptional activator activity, and that ERF34, ERF35, ERF38, and ERF39 upregulated the promoter activity ofCESA1, a PCW-typeCESAgene, through the DRECRTCOREAT elements, the core cis-acting elements known to be recognized by AP2/ERF proteins. Together, our findings show that Group IIIdERFgenes are positive transcriptional regulators of PCW-typeCESAgenes in Arabidopsis and are possibly involved in modulating cellulose biosynthesis in response to developmental requirements and environmental stimuli.