Physiological and morphological effects of overproduction of membrane‐bound ATP synthase in Escherichia coli K‐12.
Physiological and morphological effects of overproduction of membrane‐bound ATP synthase in Escherichia coli K‐12.
复制标题
大肠杆菌 K-12 中膜结合 ATP 合酶过量产生的生理和形态学影响。
DOI：
10.1002/j.1460-2075.1984.tb02047.x
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
B. Deurs
中科院分区：
文献类型：
--
作者：
K. Meyenburg;B. B. Jørgensen;B. Deurs
在生理和形态学水平上分析了大肠杆菌K‐12膜结合ATP合成酶生物合成增加的影响。通过将结构基因克隆到质粒pBR322中，实现了酶复合体的过量生产。构建了一系列质粒，分别产生2倍、4 - 5倍和10 - 12倍的过剩产量。计算出ATP合酶分别占这些质粒细胞中总蛋白的3%、6‐7%和18‐23%。在野生型细胞中，ATP合酶占总蛋白的1.5 - 2%，相当于平均每个细胞约3000个酶复合物。2 -或4 - 5倍的野生型ATP合酶水平只有轻微的影响，但发现10 - 12倍的过量生产导致细胞分裂和生长以及细胞内膜池和囊泡的形成受到明显抑制。在这些细胞中也观察到包涵体，可能代表过量ATP合酶的沉积。
Effects of increased biosynthesis of the membrane‐bound ATP synthase of Escherichia coli K‐12 were analysed at the physiological and morphological level. Overproduction of the enzyme complex was achieved by molecular cloning of the structural genes into plasmid pBR322. A series of plasmids resulting in 2‐fold, 4‐ to 5‐fold and 10‐ to 12‐fold overproduction, respectively, was constructed. The ATP synthase was calculated to represent 3%, 6‐7% and 18‐23%, respectively, of total protein in cells with these plasmids. In wild‐type cells ATP synthase represents 1.5‐2% of total protein equivalent to approximately 3000 enzyme complexes per average cell. While 2‐ or 4‐ to 5‐fold wild‐type levels of the ATP synthase had only minor effects it was found that 10‐ to 12‐fold overproduction resulted in pronounced inhibition of cell division and growth and in formation of membrane cisterns and vesicles within the cells. Inclusion bodies, probably representing deposits of excess ATP synthase, were also observed in these cells.