In situ tissue engineering with synthetic self-assembling peptide nanofiber scaffolds, PuraMatrix, for mucosal regeneration in the rat middle-ear.
In situ tissue engineering with synthetic self-assembling peptide nanofiber scaffolds, PuraMatrix, for mucosal regeneration in the rat middle-ear.
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DOI：
10.2147/ijn.s47279
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发表时间：
2013
影响因子：
8
通讯作者：
Koji T
中科院分区：
文献类型：
--
作者：
Akiyama N;Yamamoto-Fukuda T;Takahashi H;Koji T
中耳粘膜维持中耳压力。然而，大多数手术病例表现出中耳粘膜再生不足，在这种情况下，粘膜移植是必要的。本研究的目的是评估使用PuraMatrix（已成功用作组织工程支架）将分离的粘膜细胞封装在合成自组装肽支架内移植修复受损中耳的可行性。取Sprague道利（SD）转基因大鼠的中耳大疱，用增强型绿色荧光蛋白（EGFP）基因转染，切下小块，培养至第3代。在手术消除SD受体大鼠的中耳粘膜后，将供体细胞包封在PuraMatrix中并移植到这些免疫抑制大鼠中。原代培养的细胞呈泛细胞角蛋白阳性，波形蛋白阴性，保留了中耳上皮细胞的特征。在接受PuraMatrix移植后，在受体中耳中发现了高比例的EGFP表达细胞，但没有PuraMatrix。这些细胞保持正常的形态和功能，证实了组织学检查，免疫组织化学和电子显微镜，并繁殖形成新的上皮和上皮下层与基底膜。本研究证明了将培养的中耳粘膜上皮细胞包裹在PuraMatrix中移植用于SD大鼠手术切除的中耳粘膜再生的可行性。
Middle-ear mucosa maintains middle-ear pressure. However, the majority of surgical cases exhibit inadequate middle-ear mucosal regeneration, and mucosal transplantation is necessary in such cases. The aim of the present study was to assess the feasibility of transplantation of isolated mucosal cells encapsulated within synthetic self-assembling peptide nanofiber scaffolds using PuraMatrix, which has been successfully used as scaffolding in tissue engineering, for the repair of damaged middle-ear. Middle-ear bullae with mucosa were removed from Sprague Dawley (SD) transgenic rats, transfected with enhanced green fluorescent protein (EGFP) transgene and excised into small pieces, then cultured up to the third passage. After surgical elimination of middle-ear mucosa in SD recipient rats, donor cells were encapsulated within PuraMatrix and transplanted into these immunosuppressed rats. Primary cultured cells were positive for pancytokeratin but not for vimentin, and retained the character of middle-ear epithelial cells. A high proportion of EGFP-expressing cells were found in the recipient middle-ear after transplantation with PuraMatrix, but not without PuraMatrix. These cells retained normal morphology and function, as confirmed by histological examination, immunohistochemistry, and electron microscopy, and multiplied to form new epithelial and subepithelial layers together with basement membrane. The present study demonstrated the feasibility of transplantation of cultured middle-ear mucosal epithelial cells encapsulated within PuraMatrix for regeneration of surgically eliminated mucosa of the middle-ear in SD rats.