Evidence for an instructive role of retinal activity in retinotopic map refinement in the superior colliculus of the mouse
Evidence for an instructive role of retinal activity in retinotopic map refinement in the superior colliculus of the mouse
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DOI：
10.1523/jneurosci.1470-05.2005
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发表时间：
2005-07-20
影响因子：
5.3
通讯作者：
Crair, MC
中科院分区：
文献类型：
--
作者：
Chandrasekaran, AR;Plas, DT;Crair, MC
虽然它被广泛接受的分子机制在最初建立的视网膜定位图中起着重要的作用，它也一直认为，活动依赖性因素与分子机制，以改善地形图。在哺乳动物中视网膜活性在视网膜定位图细化中的作用的证据是有限的，并且关于自发视网膜活性对上级丘中感受野的发育的影响一无所知。使用解剖学和生理学方法与两个基因操纵的小鼠模型和野生型小鼠的药理学干预，我们表明，自发视网膜波指示视网膜定位图细化的上级丘的小鼠。活动依赖性机制可能在将视网膜的鼻颞轴映射到丘上的过程中发挥优先作用，因为在没有视网膜波的突变体中，沿着该轴的细化特别受损。在同一只动物中同时干扰轴突引导线索和活动依赖线索具有显著的累积效应。这些实验展示了轴突引导线索和活动依赖性因子如何联合收割机来指导视网膜定位图的开发。
Although it is widely accepted that molecular mechanisms play an important role in the initial establishment of retinotopic maps, it has also long been argued that activity-dependent factors act in concert with molecular mechanisms to refine topographic maps. Evidence of a role for retinal activity in retinotopic map refinement in mammals is limited, and nothing is known about the effect of spontaneous retinal activity on the development of receptive fields in the superior colliculus. Using anatomical and physiological methods with two genetically manipulated mouse models and pharmacological interventions in wild-type mice, we show that spontaneous retinal waves instruct retinotopic map refinement in the superior colliculus of the mouse. Activity-dependent mechanisms may play a preferential role in the mapping of the nasal-temporal axis of the retina onto the colliculus, because refinement is particularly impaired along this axis in mutants without retinal waves. Interfering with both axon guidance cues and activity-dependent cues in the same animal has a dramatic cumulative effect. These experiments demonstrate how axon guidance cues and activity-dependent factors combine to instruct retinotopic map development.