Extracorporeal photopheresis normalizes some lymphocyte subsets (including T regulatory cells) in chronic graft-versus-host-disease
Extracorporeal photopheresis normalizes some lymphocyte subsets (including T regulatory cells) in chronic graft-versus-host-disease
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DOI：
10.1111/j.1744-9987.2008.00593.x
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发表时间：
2008-08-01
影响因子：
1.9
通讯作者：
Taylor, Peter
中科院分区：
文献类型：
--
作者：
Bladon, John;Taylor, Peter
体外光采术（ECP）已被证明在临床上可有效治疗类固醇难治性移植物抗宿主病（GvHD）。最近，小鼠模型表明 ECP 可能通过增强 T 调节细胞来调节 GvHD 中的同种异体反应性。 T 调节细胞是免疫耐受的重要组成部分，在慢性 GvHD 中观察到 T 调节细胞水平降低。在接受任何 ECP 治疗前和 ECP 治疗三个月后，从八名慢性 GvHD 患者身上采集样本。样本还取自八名年龄和性别匹配的正常对照。评估每个样本的总淋巴细胞以及CD4(+)和CD8(+)T细胞的绝对水平。还相应地评估了T调节细胞数量：CD4(+)CD25(高)、CD4(+)CD25(高)CD69(-)、CD4(+)CD25(高)CD152(+)和CD4(+)CD25(高)FOXP3(+)。三个月的ECP后，观察到总淋巴细胞、CD4(+)和CD8(+) T细胞以及T调节细胞的绝对计数增加；然而，在 CD4(+) T 细胞室内没有观察到 T 调节细胞数量百分比的选择性统计增加。 ECP 诱导 T 调节细胞数量增加；然而，这并不具体，因为总淋巴细胞和 CD4(+) T 细胞数量也有所增加。因此，ECP 的积极作用可能取决于对免疫稳态的更普遍的重新调整。
Extracorporeal photopheresis (ECP) has been demonstrated to be clinically effective for the treatment of steroid-refractory graft-versus-host-disease (GvHD). Recently, a murine model suggested that ECP may modulate the allo-reactivity, seen in GvHD, by enhancement of T regulatory cells. T regulatory cells are an important component of immune tolerance and reduced levels of them have been observed in chronic GvHD. Samples were taken from eight chronic GvHD patients prior to receiving any ECP therapy and after three months of ECP. Samples were also obtained from eight age- and sex-matched normal controls. Each sample was evaluated for absolute levels of total lymphocytes and CD4(+) and CD8(+) T cells. T regulatory cell numbers were also assessed accordingly: CD4(+)CD25(high), CD4(+)CD25(high)CD69(-), CD4(+)CD25(high)CD152(+) and CD4(+)CD25(high)FOXP3(+). Following three months of ECP, increases in the absolute counts of total lymphocytes, CD4(+) and CD8(+) T cells and T regulatory cells were observed; however, no selective statistical increase in the percentage of T regulatory cell numbers was observed within the CD4(+) T cell compartment. ECP induces an increase in T regulatory cell numbers; however, this is not specific as there is also an enhancement of both total lymphocyte and CD4(+) T cell numbers. The positive effect of ECP may therefore depend on a more generic re-adjustment of immune homeostasis.