Regulation of the swarming inhibitor disA in Proteus mirabilis.
Regulation of the swarming inhibitor disA in Proteus mirabilis.
复制标题
奇异变形杆菌中群聚抑制剂 disA 的调节。
DOI：
10.1128/jb.00039-13
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发表时间：
2013
影响因子：
3.2
通讯作者：
Rather,PhilipN
中科院分区：
文献类型：
--
作者：
Szostek,BreeA;Rather,PhilipN
disA基因编码一种假定的氨基酸脱羧酶，该酶抑制奇异变形杆菌的群集。5′端cDNA末端快速扩增（RACE）和缺失分析鉴定disA启动子。adisA-lacZ融合的使用表明，FlhD 4C 2，一类鞭毛主调节因子，在失调中没有作用。DisA的推定产物苯乙胺能够抑制DisA的表达，表明存在负调节反馈环。利用转座子突变技术对disA的调控因子进行了鉴定，发现umoB（igaA）是disA的负调控因子。我们的数据表明UmoB对disA的调节是通过Rcs磷酸化介导的。
ThedisAgene encodes a putative amino acid decarboxylase that inhibits swarming in Proteus mirabilis. 5′ rapid amplification of cDNA ends (RACE) and deletion analysis were used to identify thedisApromoter. The use of adisA-lacZfusion indicated that FlhD4C2, the class I flagellar master regulator, did not have a role indisAregulation. The putative product of DisA, phenethylamine, was able to inhibitdisAexpression, indicating that a negative regulatory feedback loop was present. Transposon mutagenesis was used to identify regulators ofdisAand revealed thatumoB(igaA) was a negative regulator ofdisA. Our data demonstrate that the regulation ofdisAby UmoB is mediated through the Rcs phosphorelay.