Elevated plus maze for mice.
Elevated plus maze for mice.
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DOI：
10.3791/1088
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发表时间：
2008-12-22
期刊：
影响因子：
--
通讯作者：
Miyakawa, Tsuyoshi
中科院分区：
文献类型：
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作者：
Komada, Munekazu;Takao, Keizo;Miyakawa, Tsuyoshi
虽然小鼠基因组现已完全测序，但大脑中表达的大多数基因的功能尚不清楚。特定基因对特定行为的影响可以通过突变小鼠的行为分析来确定。如果目的基因在脑中表达，突变小鼠的行为表型可以阐明正常行为的遗传机制。高架十字迷宫测试是用于测量焦虑样行为的最广泛使用的测试之一。该测试基于小鼠对开放和高架区域的自然厌恶，以及它们在新环境中的自然自发探索行为。该装置由中间相互垂直交叉的开放臂和闭合臂以及中心区域组成。小鼠可以接触所有手臂，并可以在手臂之间自由移动。进入开放臂的次数和在开放臂中花费的时间被用作小鼠中开放空间诱导的焦虑的指标。不幸的是，实验室之间存在的程序差异使得难以复制和比较实验室之间的结果。在这里，我们提出了一个详细的电影演示我们的协议高架十字迷宫测试。在我们的实验室中，我们已经使用电影中显示的协议评估了90多个突变小鼠品系。这些数据将作为我们正在建设的公共数据库的一部分公布。可视化的协议将促进更好地理解整个实验程序的细节，允许在不同的实验室中使用的协议的标准化和使用该测试评估的各种品系的突变小鼠的行为表型的比较。
Although the mouse genome is now completely sequenced, the functions of most of the genes expressed in the brain are not known. The influence of a given gene on a specific behavior can be determined by behavioral analysis of mutant mice. If a target gene is expressed in the brain, behavioral phenotype of the mutant mice could elucidate the genetic mechanism of normal behaviors. The elevated plus maze test is one of the most widely used tests for measuring anxiety-like behavior. The test is based on the natural aversion of mice for open and elevated areas, as well as on their natural spontaneous exploratory behavior in novel environments. The apparatus consists of open arms and closed arms, crossed in the middle perpendicularly to each other, and a center area. Mice are given access to all of the arms and are allowed to move freely between them. The number of entries into the open arms and the time spent in the open arms are used as indices of open space-induced anxiety in mice. Unfortunately, the procedural differences that exist between laboratories make it difficult to duplicate and compare results among laboratories. Here, we present a detailed movie demonstrating our protocol for the elevated plus maze test. In our laboratory, we have assessed more than 90 strains of mutant mice using the protocol shown in the movie. These data will be disclosed as a part of a public database that we are now constructing. Visualization of the protocol will promote better understanding of the details of the entire experimental procedure, allowing for standardization of the protocols used in different laboratories and comparisons of the behavioral phenotypes of various strains of mutant mice assessed using this test.