Natural killer cell activation after infection with lactate dehydrogenase-elevating virus
Natural killer cell activation after infection with lactate dehydrogenase-elevating virus
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DOI：
10.1099/0022-1317-83-11-2709
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发表时间：
2002-11-01
影响因子：
3.8
通讯作者：
Coutelier, JP
中科院分区：
文献类型：
--
作者：
Markine-Goriaynoff, D;Hulhoven, X;Coutelier, JP
感染后早期，乳酸脱氢酶升高病毒（LDV）通过多克隆激活B淋巴细胞（导致IgG 2a限制性高丙种球蛋白血症）和抑制Th 2细胞因子的分泌来改变免疫系统。考虑到这些改变可能涉及先天免疫系统的细胞和细胞因子，如干扰素-γ（IFN-γ），我们分析了LDV对自然杀伤（NK）细胞的影响。在感染的几天内，观察到强烈和短暂的NK细胞活化，其特征在于增强的IFN-γ信息表达和细胞溶解。LDV引发血清IFN-γ水平的大幅增加。由于NK细胞和IFN-γ可能参与防御病毒感染，我们分析了它们可能的作用，在控制LDV滴度与一个新的凝集试验。我们的研究结果表明，无论是NK细胞的激活，也没有IFN-γ的分泌影响早期和快速的病毒复制，LDV接种后。
Early after infection, lactate dehydrogenase-elevating virus (LDV) alters the immune system by polyclonally activating B lymphocytes, which leads to IgG2a-restricted hypergammaglobulinaemia, and by suppressing the secretion of Th2 cytokines. Considering that these alterations may involve cells of the innate immune system and cytokines such as interferon-gamma (IFN-gamma), we analysed the effect of LDV on natural killer (NK) cells. Within a few days of infection, a strong and transient NK cell activation, characterized by enhanced IFN-gamma message expression and cytolysis, was observed. LDV triggered a large increase in serum IFN-gamma levels. Because NK cells and IFN-gamma may participate in the defence against virus infection, we analysed their possible role in the control of LDV titres with a new agglutination assay. Our results indicate that neither the activation of NK cells nor the IFN-gamma secretion affect the early and rapid virus replication that follows LDV inoculation.