Biolens behavior of RBCs under optically‐induced mechanical stress
Biolens behavior of RBCs under optically‐induced mechanical stress
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DOI：
10.1002/cyto.a.23085
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发表时间：
2017-05
期刊：
影响因子：
3.7
通讯作者：
F. Merola;Á. Barroso;L. Miccio;P. Memmolo;M. Mugnano;P. Ferraro;C. Denz
中科院分区：
文献类型：
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作者：
F. Merola;Á. Barroso;L. Miccio;P. Memmolo;M. Mugnano;P. Ferraro;C. Denz
在这项工作中，研究了红细胞（RBC）在光致机械应力下的光学行为。利用关于RBC的光学透镜样行为的新发现，进一步研究了由光学变形RBC折射的波前的变化。采用数字全息术和基于Zernike多项式的数值分析相结合的方法，对生物透镜在多个动态光镊作用下的变形进行了实验分析。详细的波前分析提供了关于由所施加的机械应力引起的像差的全面信息。通过这种方法，它表明，红细胞在其盘状细胞形式的光学性质可以以不同的方式取决于变形的几何形状的影响。类似于经典的光学测试过程，光学参数可以与特定的机械变形相关联。由于干涉仪工具的高灵敏度，这可以通过检查光学参数而不是直接但具有低分辨率的应变分析来打开分析细胞弹性的新路线。这种方法的未来应用可以通过单步波前分析来评估不同细胞的弹性，从而早期检测和诊断血液疾病。© 2017国际细胞计数促进学会
In this work, the optical behavior of Red Blood Cells (RBCs) under an optically‐induced mechanical stress was studied. Exploiting the new findings concerning the optical lens‐like behavior of RBCs, the variations of the wavefront refracted by optically‐deformed RBCs were further investigated. Experimental analysis have been performed through the combination of digital holography and numerical analysis based on Zernike polynomials, while the biological lens is deformed under the action of multiple dynamic optical tweezers. Detailed wavefront analysis provides comprehensive information about the aberrations induced by the applied mechanical stress. By this approach it was shown that the optical properties of RBCs in their discocyte form can be affected in a different way depending on the geometry of the deformation. In analogy to classical optical testing procedures, optical parameters can be correlated to a particular mechanical deformation. This could open new routes for analyzing cell elasticity by examining optical parameters instead of direct but with low resolution strain analysis, thanks to the high sensitivity of the interferometric tool. Future application of this approach could lead to early detection and diagnosis of blood diseases through a single‐step wavefront analysis for evaluating different cells elasticity. © 2017 International Society for Advancement of Cytometry