Disruption of diapause induction by TALEN-based gene mutagenesis in relation to a unique neuropeptide signaling pathway in Bombyx.
Disruption of diapause induction by TALEN-based gene mutagenesis in relation to a unique neuropeptide signaling pathway in Bombyx.
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DOI：
10.1038/srep15566
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发表时间：
2015-10-26
影响因子：
4.6
通讯作者：
Mizoguchi A
中科院分区：
文献类型：
--
作者：
Shiomi K;Takasu Y;Kunii M;Tsuchiya R;Mukaida M;Kobayashi M;Sezutsu H;Ichida Takahama M;Mizoguchi A
昆虫神经肽家族 FXPRLa 在 C 末端携带 Phe-Xaa-Pro-Arg-Leu-NH2 序列，参与许多生理过程。尽管 FXPRLa 信号传导中配体-受体相互作用已通过体外测定进行了检查，但这些相互作用与体内生理功能之间的相关性尚不清楚。家蚕 Bombyx mori 的滞育被认为是由滞育激素（DH，FXPRLa）信号传导引起的，该信号传导由 DH 和 DH 受体 (DHR) 之间的相互作用组成。在这里，我们对家蚕 DH-PBAN 和 DHR 基因进行了基于转录激活子样效应核酸酶 (TALEN) 的诱变，并在二化菌株中分离了这些基因的无效突变体。所有突变蚕均完全存活，并且蜕皮或变态的发育时间没有异常。然而，尽管存在诱导滞育的温度和光周期条件，雌性成虫仍产下非滞育卵。因此，我们得出结论，DH 信号传导对于滞育诱导至关重要，并且由家蚕配体-受体共同进化过程中选择的 DH 和 DHR 之间的高度敏感和特异性相互作用组成。
The insect neuropeptide family FXPRLa, which carries the Phe-Xaa-Pro-Arg-Leu-NH2 sequence at the C-terminus, is involved in many physiological processes. Although ligand–receptor interactions in FXPRLa signaling have been examined using in vitro assays, the correlation between these interactions and in vivo physiological function is unclear. Diapause in the silkworm, Bombyx mori, is thought to be elicited by diapause hormone (DH, an FXPRLa) signaling, which consists of interactions between DH and DH receptor (DHR). Here, we performed transcription activator-like effector nuclease (TALEN)-based mutagenesis of the Bombyx DH-PBAN and DHR genes and isolated the null mutants of these genes in a bivoltine strain. All mutant silkworms were fully viable and showed no abnormalities in the developmental timing of ecdysis or metamorphosis. However, female adults oviposited non-diapause eggs despite diapause-inducing temperature and photoperiod conditions. Therefore, we conclude that DH signaling is essential for diapause induction and consists of highly sensitive and specific interactions between DH and DHR selected during ligand–receptor coevolution in Bombyx mori.