AN INHIBITION ENZYME-IMMUNOASSAY FOR ESTIMATING RELATIVE ANTIBODY-AFFINITY AND AFFINITY HETEROGENEITY
AN INHIBITION ENZYME-IMMUNOASSAY FOR ESTIMATING RELATIVE ANTIBODY-AFFINITY AND AFFINITY HETEROGENEITY
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DOI：
10.1016/0022-1759(88)90204-9
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发表时间：
1988-02-10
影响因子：
2.2
通讯作者：
STEWARD, MW
中科院分区：
文献类型：
--
作者：
RATH, S;STANLEY, CM;STEWARD, MW
描述了一种使用抑制酶免疫测定法测量抗体相对亲和力的方法。使用具有确定亲和力特征的单克隆抗体并通过与常规亲和力测量方法进行比较来验证。该方法允许测量低水平抗体的相对亲和力，并计算抗体群体中亲和力异质性的经验估计值。
A method to measure the relative affinity of antibodies using an inhibition enzyme immunoassay is described. It is validated using monoclonal antibodies of defined affinity characteristics and by comparison with conventional methods of affinity measurement. The method allows measurement of the relative affinity of low levels of antibody, and the calculation of an empirical estimate of the heterogeneity of affinity in antibody populations.