Production of very-high-amylose cassava by post-transcriptional silencing of branching enzyme genes.
Production of very-high-amylose cassava by post-transcriptional silencing of branching enzyme genes.
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通过分支酶基因的转录后沉默生产极高直链淀粉木薯。
DOI：
10.1111/jipb.12848
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发表时间：
2020
影响因子：
11.4
通讯作者：
Peng Zhang
中科院分区：
文献类型：
--
作者：
Wenzhi Zhou;Shanshan Zhao;Shutao He;Qiuxiang Ma;Xinlu Lu;Xiaomeng Hao;Hongxia Wang;Jun Yang;Peng Zhang
高直链淀粉可以由分支酶（BE）功能缺陷的植物产生。在这里，我们报告的生产转基因木薯与淀粉含有高达50%的直链淀粉由于组成型表达的发夹双链RNA靶向BE 1或BE 2基因。所有BE 1-RNAi植物系（BE 1 i）和BE 2-RNAi植物系（BE 2 i）都在田间生长，但总生物量产量降低。在BE 2 i植物系的贮藏根中实现了相当高的直链淀粉含量。BE 1 i和BE 2 i植株的贮藏淀粉粒形态与野生型相似，但BE 1 i植株的贮藏淀粉粒比野生型大。所有三种来源的储存淀粉的淀粉谱图和热谱图的比较揭示了BE 2 i淀粉的糊化特性和更高的熔融温度的显著变化。葡聚糖链长分布分析显示，在BE 1 i系中DP>36的链略有增加，在BE 2 i系中DP 10-20和DP>40之间的葡聚糖链显著增加。此外，BE 2 i淀粉显示出B型X射线衍射图，而不是BE 1 i和WT淀粉中发现的A型图。因此，木薯BE 1和BE 2在贮藏根淀粉合成中的功能不同。本文受版权保护。All rights reserved.
High amylose starch can be produced by plants deficient in the function of branching enzymes (BEs). Here we report the production of transgenic cassava with starches containing up to 50% amylose due to the constitutive expression of hair-pin dsRNAs targeting the BE1 or BE2 genes. All BE1-RNAi plant lines (BE1i) and BE2-RNAi plant lines (BE2i) were grown up in the field, but with reduced total biomass production. Considerably high amylose content in the storage roots of BE2i plant lines was achieved. Storage starch granules of BE1i and BE2i plants had similar morphology as wild type (WT), however, the size of BE1i starch granules were bigger than that of WT. Comparisons of amylograms and thermograms of all three sources of storage starches revealed dramatic changes to the pasting properties and a higher melting temperature for BE2i starches. Glucan chain length distribution analysis showed a slight increase in chains of DP>36 in BE1i lines and a dramatic increase in glucan chains between DP 10-20 and DP>40 in BE2i lines. Furthermore, BE2i starches displayed a B-type X-ray diffraction pattern instead of the A-type pattern found in BE1i and WT starches. Therefore, cassava BE1 and BE2 function differently in storage root starch biosynthesis. This article is protected by copyright. All rights reserved.