Cooperation between different RNA virus genomes produces a new phenotype
Cooperation between different RNA virus genomes produces a new phenotype
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DOI：
10.1038/ncomms2252
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发表时间：
2012-12-01
影响因子：
16.6
通讯作者：
Yanagi, Yusuke
中科院分区：
文献类型：
--
作者：
Shirogane, Yuta;Watanabe, Shumpei;Yanagi, Yusuke
由于病毒RNA聚合酶的高错误率，RNA病毒群体通常在选择压力下快速进化。麻疹病毒是一种包膜RNA病毒，具有一种介导病毒包膜与细胞膜融合的融合蛋白。在这里，我们观察到非融合性重组麻疹病毒在传代后进化成突变病毒，突变病毒重新获得诱导膜融合的能力。出乎意料的是，我们在一个病毒粒子中发现了一种突变病毒，它具有两种类型的基因组：一种基因组编码野生型融合蛋白，另一种是具有单个氨基酸替代的突变版本。野生型和突变型蛋白本身都不能介导膜融合，但两者通过异齐聚物的形成共同表现出增强的融合活性。我们的研究结果揭示了不同RNA病毒基因组之间“合作”的分子机制，这可能对病毒进化和其他大分子的进化有影响。
An RNA virus population generally evolves rapidly under selection pressure, because of high error rates of the viral RNA polymerase. Measles virus, an enveloped RNA virus, has a fusion protein mediating fusion of the viral envelope with the cell membrane. Here we observe that a non-fusogenic recombinant measles virus evolves, after passages, into mutant viruses which regain the ability to induce membrane fusion. Unexpectedly, we identify a mutant virus possessing two types of genomes within a single virion: one genome encoding the wild-type fusion protein, the other a mutant version with a single amino-acid substitution. Neither the wild-type nor mutant protein by itself is able to mediate membrane fusion, but both together exhibit enhanced fusion activity through hetero-oligomer formation. Our results reveal a molecular mechanism for the 'cooperation' between different RNA virus genomes, which may have implications in viral evolution and in the evolution of other macromolecules.