Synergistic effects of the combined use of human-cultured periosteal sheets and platelet-rich fibrin on bone regeneration: An animal study.
Synergistic effects of the combined use of human-cultured periosteal sheets and platelet-rich fibrin on bone regeneration: An animal study.
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DOI：
10.1002/cre2.71
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发表时间：
2017-08
影响因子：
1.8
通讯作者：
Yoshie H
中科院分区：
文献类型：
--
作者：
Horimizu M;Kubota T;Kawase T;Nagata M;Kobayashi M;Okuda K;Nakata K;Yoshie H
人类培养的牙槽骨源性骨膜（hCP）片是临床上用于牙周再生治疗的一种植骨材料，而富血小板纤维蛋白（PRF），一种含有纤维蛋白凝块的血小板浓缩物，被认为可以促进伤口愈合过程。因此，我们在动物模型中评估了hCP - PRF复合物联合使用是否能协同促进骨再生。人骨膜段（1 × 1 mm）在塑料培养皿上初始培养，形成hCP片。将新鲜制备的人PRF植入裸鼠皮下组织（hCP: n = 4, hCP + PRF: n = 4）和4mm直径颅骨骨缺损模型（hCP: n = 4, hCP + PRF: n = 4，对照[缺陷]:n = 4）。植骨后4周，采用μCT观察骨形成情况。用组织化学和免疫组织学方法评价细胞生长和新生血管的形成。在皮下组织中，hCP + PRF组的矿物质沉积、胶原沉积和血管数量均高于单独hCP组。在颅骨缺损模型中，hCP + PRF组的新骨形成明显高于hCP单独组和仅缺损对照组。与单独使用hCP组相比，hCP + PRF组颅骨缺损中血管和PCNA阳性细胞的数量也有所增加。富含血小板的纤维蛋白制剂支持骨膜细胞的增殖和生长，形成良好结合的活性生物材料。富含血小板的纤维蛋白也刺激着床部位的局部血管生成。因此，hCP与PRF联合应用在骨再生治疗中具有临床应用价值。
A human‐cultured alveolar bone‐derived periosteal (hCP) sheet is an osteogenic grafting material used clinically in periodontal regenerative therapy, while platelet‐rich fibrin (PRF), a platelet concentrate with fibrin clot, is considered to augment the wound healing process. Therefore, whether the combined use of hCP‐PRF complex could facilitate bone regeneration synergistically was evaluated in animal models. Human periosteal segments (1 × 1 mm) were cultured initially on plastic dishes and formed an hCP sheet. The hCP sheet was implanted with freshly prepared human PRF into subcutaneous tissue (hCP: n = 4, hCP + PRF: n = 4) and 4 mm diameter calvarial bone defect models (hCP: n = 4, hCP + PRF: n = 4, control [defect‐only]: n = 4) that prepared in nude mice. At 4 weeks postimplantation, new bone formation was evaluated by using μCT. Cell growth and neovascularization were evaluated by histochemical and immunohistological methods. In the subcutaneous tissue, mineral deposit formation, collagen deposition, and number of vessels were higher in the hCP + PRF group than in the hCP alone group. In the calvarial defect models, new bone formation was significantly higher in the hCP + PRF group than in the hCP alone group and defect‐only control group. The numbers of vessels and PCNA‐positive cells in calvarial defects were also increased in the hCP + PRF group more than in the hCP alone group. Platelet‐rich fibrin preparations support the proliferation and the growth of periosteal cells to form well‐combined active biological materials. Platelet‐rich fibrin also stimulates the local angiogenesis in the implantation site. Therefore, the combined use of hCP and PRF could be clinically applicable in bone regeneration therapy.