Quantitative Lipoprotein Subclass and Low Molecular Weight Metabolite Analysis in Human Serum and Plasma by 1H NMR Spectroscopy in a Multilaboratory Trial
Quantitative Lipoprotein Subclass and Low Molecular Weight Metabolite Analysis in Human Serum and Plasma by 1H NMR Spectroscopy in a Multilaboratory Trial
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DOI：
10.1021/acs.analchem.8b02412
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发表时间：
2018-10-16
影响因子：
7.4
通讯作者：
Nicholson, Jeremy K.
中科院分区：
文献类型：
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作者：
Jimenez, Beatriz;Holmes, Elaine;Nicholson, Jeremy K.
我们报告了一个广泛的600 MHz核磁共振试验定量脂蛋白和小分子测量在人类血清和血浆。使用5个中心的11台600 MHz NMR光谱仪分析了98份样本，包括20份质控品（QC）、37份市售配对血清和血浆样本以及2份美国国家科学技术研究院（NIST）参比物质1951 c重复样品。使用严格的样品制备和实验采集方案分析样品。一个商业脂蛋白亚类分析被用来量化105脂蛋白亚类和24个低分子量代谢产物的NMR光谱。对于所有光谱仪，测量内部QC的仪器特定方差低于国家胆固醇教育计划（NCEP）脂质检测标准（甘油三酯）所描述的百分比
We report an extensive 600 MHz NMR trial of quantitative lipoprotein and small-molecule measurements in human blood serum and plasma. Five centers with eleven 600 MHz NMR spectrometers were used to analyze 98 samples including 20 quality controls (QCs), 37 commercially sourced, paired serum and plasma samples, and two National Institute of Science and Technology (NIST) reference material 1951c replicates. Samples were analyzed using rigorous protocols for sample preparation and experimental acquisition. A commercial lipoprotein subclass analysis was used to quantify 105 lipoprotein subclasses and 24 low molecular weight metabolites from the NMR spectra. For all spectrometers, the instrument specific variance in measuring internal QCs was lower than the percentage described by the National Cholesterol Education Program (NCEP) criteria for lipid testing [triglycerides