RpS3, a DNA repair endonuclease and ribosomal protein, is involved in apoptosis
RpS3, a DNA repair endonuclease and ribosomal protein, is involved in apoptosis
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DOI：
10.1016/s0014-5793(04)00074-2
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发表时间：
2004-02-27
期刊：
影响因子：
3.5
通讯作者：
Kim, J
中科院分区：
文献类型：
--
作者：
Jang, CY;Lee, JY;Kim, J
已知哺乳动物rpS 3作为DNA修复内切核酸酶和核糖体蛋白S3发挥功能。还观察到几种核糖体蛋白或DNA修复酶与细胞凋亡有关。我们在这里报告了rpS 3的第三个功能，即诱导细胞凋亡。当淋巴细胞经历rpS 3诱导的凋亡时，绿色荧光蛋白（GFP）-rpS 3的定位改变到核膜。GFP-rpS 3的瞬时表达激活caspase-8/caspase-3并使苦参碱诱导的细胞凋亡增敏。缺失分析表明，rpS 3的两个功能，DNA修复和凋亡，使用独立的功能域。(C)2004年欧洲生物化学学会联合会。Elsevier B. V.出版，保留所有权利。
It is known that mammalian rpS3 functions as a DNA repair endonuclease and ribosomal protein S3. It was also observed that several ribosomal proteins or DNA repair enzymes are related to apoptosis. We report here a third function of rpS3, induction of apoptosis. The localization of green fluorescent protein (GFP)-rpS3 is changed to the nuclear membrane when lymphocytic cells undergo rpS3-induced apoptosis. Transient expression of GFP-rpS3 activates caspase-8/caspase-3 and sensitizes cytokine-induced apoptosis. Deletion analysis reveals that the two functions of rpS3, DNA repair and apoptosis, use independent functional domains. (C) 2004 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.