Complementary deoxyribonucleic acid cloning of spermatogonial stem cell renewal factor
Complementary deoxyribonucleic acid cloning of spermatogonial stem cell renewal factor
复制标题
DOI：
10.1210/en.2003-0800
复制
发表时间：
2003-12-01
期刊：
影响因子：
4.8
通讯作者：
Yamauchi, K
中科院分区：
文献类型：
--
作者：
Miura, T;Ohta, T;Yamauchi, K
精原细胞有丝分裂可分为精原干细胞更新和精原细胞向减数分裂方向增殖两个过程。最近研究表明雌激素E2 - 17 β参与调控鳗鱼精原干细胞的更新。为了确定直接调节这一过程的基因，我们使用表达筛选来鉴定睾丸中雌二醇-17 β调节其表达的基因。我们检测到一个先前未鉴定的cDNA克隆，该克隆被雌二醇-17 β刺激上调，并将其命名为鳗鱼精子发生相关物质34（eSRS 34）cDNA。同源性检索显示eSRS 34与人血小板源性内皮细胞生长因子具有相似的氨基酸序列。我们使用几种体外系统检查了eSRS 34的功能。杆状病毒系统产生的重组eSRS 34在睾丸器官培养中诱导精原细胞有丝分裂。此外，在生殖细胞/体细胞共培养系统中，添加eSRS 34特异性抗体可阻止雌二醇-17 β刺激诱导的精原细胞有丝分裂。因此，我们得出结论，eSRS 34是一种“精原干细胞更新因子”。"
Spermatogonial mitosis can be subdivided into two processes: spermatogonial stem cell renewal and spermatogonial proliferation toward meiosis. Recently it has been indicated that estrogen, estradiol-17beta, is involved in regulating the renewal of spermatogonial stem cells in eel. To determine the genes that directly regulate this process, we used expression screening to identify genes whose expression is regulated by estradiol-17beta in testes. We detected a previously unidentified cDNA clone that is up-regulated by estradiol-17beta stimulation and named it eel spermatogenesis-related substances 34 (eSRS34) cDNA. Homology searching showed that eSRS34 shares amino acid sequence similarity with human platelet-derived endothelial cell growth factor. We examined the function of eSRS34 using several in vitro systems. Recombinant eSRS34 produced by a baculovirus system induced spermatogonial mitosis in testicular organ culture. Furthermore, the addition of an antibody specific for eSRS34 prevented spermatogonial mitosis induced by estradiol-17beta stimulation in a germ cell/somatic cell coculture system. We therefore conclude that eSRS34 is a "spermatogonial stem cell renewal factor."