Innovative approaches to vitamin A assessment
Innovative approaches to vitamin A assessment
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DOI：
10.1093/jn/131.5.1626s
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发表时间：
2001-05-01
影响因子：
4.2
通讯作者：
Craft, NE
中科院分区：
文献类型：
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作者：
Craft, NE
由于缺乏维生素a，每年有数百万儿童的健康和视力受到损害。血清视黄醇是VA状态最常用的指标。不幸的是，在国家调查中使用它是不切实际的，因为它涉及静脉血的收集、离心和分析前的冷冻储存。为了使VA评估更实用，我们开发了结合干血点（DBS）或便携式仪器的方法。脑起搏器已被用作筛选新生儿许多生化化合物的样本基质。直到最近，人们才认为VA在DBS中是不稳定的。然而，我们证明了DBS视黄醇的测量与健康成年人（r(2) = 0.88-0.90）和受损人群（r(2) = 0.73-0.84）的血清视黄醇相关。与血清视黄醇相比，DBS视黄醇检测VA缺乏症的敏感性为73 ~ 93%，特异性为90 ~ 100%。虽然数据很少，但视黄醇结合蛋白（RBP）也可以在DBS中测量。REP已被用作血清视黄醇的替代标志物。血清REP与血清视黄醇的相关系数（r(2)）为0.4 ~ 0.8。此外，还进行了开发便携式仪器的工作，以测量现场的VA状态。对VA荧光荧光仪进行了优化，并在直接荧光测量血清全rbp的缺陷范围内呈线性。使用该仪器直接测量一滴全血中的全息rbp正在取得进展。
The health and sight of millions of children are compromised each year as a consequence of vitamin A (VA) deficiency. Serum retinol is the most commonly used indicator of VA status. Unfortunately, its use is impractical for national surveys because it involves collection of venous blood, centrifugation and frozen storage before analysis. To make VA assessment more practical, we have developed approaches incorporating dried blood spots (DBS) or portable instrumentation. DBS have been used as a sample matrix to screen neonates for many biochemical compounds. Until recently, it was not thought that VA was stable in DBS, However, we demonstrated that the measure of DBS retinol correlates well with serum retinol in both healthy adults (r(2) = 0.88-0.90) and compromised populations (r(2) = 0.73-0.84). Compared with serum retinol, the sensitivity and specificity of detecting VA deficiency by DBS retinol range from 73 to 93% and from 90 to 100%, respectively. Although few data are available, retinol binding protein (RBP) can also be measured in DBS. REP has been used as a surrogate marker for serum retinol. Correlations coefficients (r(2)) between serum REP and serum retinol range from 0.4 to 0.8. in addition, work has been done to develop portable instrumentation to measure VA status in the field. A fluorometer has been optimized for VA fluorescence and is linear into the deficient range for the direct fluorimetric measurement of serum holo-RBP. Progress is being made to use the instrument to directly measure holo-RBP in a drop of whole blood.