Genes associated with inflammation and bone remodeling are highly expressed in the bone of patients with the early-stage cam-type femoroacetabular impingement.
Genes associated with inflammation and bone remodeling are highly expressed in the bone of patients with the early-stage cam-type femoroacetabular impingement.
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DOI：
10.1186/s13018-021-02499-y
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发表时间：
2021-05-29
影响因子：
2.6
通讯作者：
Xu Y
中科院分区：
文献类型：
--
作者：
Gao G;Wu R;Liu R;Wang J;Ao Y;Xu Y
最近的研究表明，某些炎症，合成代谢和分解代谢基因的高表达水平的髋关节撞击区与股骨髋臼撞击（FAI），代表了增加的代谢状态。然而，对FAI髋关节撞击区骨组织的分子特性知之甚少。在髋关节镜手术治疗凸轮型FAI期间，采集早期凸轮型FAI患者的骨组织样本。对照骨组织样本采集自6例因股骨颈骨折接受全髋关节置换术的患者。定量实时聚合酶链反应（PCR）进行，以确定与炎症和骨重建相关的基因表达。根据临床参数评估早期凸轮型FAI患者骨组织中基因表达的差异。总共有12例早期凸轮型FAI患者和6例对照组患者被纳入本研究。FAI患者骨组织中IL-6、ALP、RANKL和OPG的表达水平均高于对照组（P < 0.05）。仅在对照组中检测到IL-1表达。另一方面，FAI患者与对照组之间IL-8的表达无显著差异。FAI患者中，体重指数（BMI）>24 kg/m2者ALP表达明显高于BMI>24 kg/m2者（P < 0.05）。外侧中心-边缘角>30°的FAI患者IL-6和ALP表达明显高于外侧中心-边缘角>30°的FAI患者（P < 0.05）。我们的结果表明，早期凸轮型FAI患者的骨组织代谢状况不同于正常骨的股骨头颈交界处。早期凸轮型FAI患者骨组织中与炎症和骨重建相关的基因表达水平高于正常骨组织。
Recent studies have shown high expression levels of certain inflammatory, anabolic, and catabolic genes in the articular cartilage from the impingement zone of the hips with femoroacetabular impingement (FAI), representing an increased metabolic state. Nevertheless, little is known about the molecular properties of bone tissue from the impingement zone of hips with FAI. Bone tissue samples from patients with early-stage cam-type FAI were collected during hip arthroscopy for treatment of cam-type FAI. Control bone tissue samples were collected from six patients who underwent total hip replacement because of a femoral neck fracture. Quantitative real-time polymerase chain reaction (PCR) was performed to determine the gene expression associated with inflammation and bone remodeling. The differences in the gene expression in bone tissues from the patients with early-stage cam-type FAI were also evaluated based on clinical parameters. In all, 12 patients with early-stage cam-type FAI and six patients in the control group were included in this study. Compared to the control samples, the bone tissue samples from patients with FAI showed higher expression levels of interleukin-6 (IL-6), alkaline phosphatase (ALP), receptor activator of nuclear factor-kB ligand (RANKL), and osteoprotegerin (OPG) (P < 0.05). IL-1 expression was detected only in the control group. On the other hand, there was no significant difference in IL-8 expression between the patients with FAI and the control group. The patients with FAI having a body mass index (BMI) of >24 kg/m2 showed higher ALP expression (P < 0.05). Further, the expression of IL-6 and ALP was higher in the patients with FAI in whom the lateral center-edge angle was >30° (P < 0.05). Our results indicated the metabolic condition of bone tissues in patients with early-stage cam-type FAI differed from that of normal bone in the femoral head-neck junction. The expression levels of the genes associated with inflammation and bone remodeling were higher in the bone tissue of patients with early-stage cam-type FAI than in the patients with normal bone tissue.
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影响因子：
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作者：
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DOI：
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影响因子：
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