emo-1, a Caenorhabditis elegans Sec61p gamma homologue, is required for oocyte development and ovulation.
emo-1, a Caenorhabditis elegans Sec61p gamma homologue, is required for oocyte development and ovulation.
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DOI：
10.1083/jcb.134.3.699
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发表时间：
1996-08
期刊：
影响因子：
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通讯作者：
Schedl T
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文献类型：
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作者：
Iwasaki K;McCarter J;Francis R;Schedl T
emo-1（oz 1）是秀丽隐杆线虫中一类雌雄同体不育突变的成员，可在性腺臂中产生内有丝分裂卵母细胞。emo-1（oz 1）突变体中的卵母细胞在卵子发生过程中表现出多种缺陷。减数分裂成熟后，排卵失败，卵母细胞被困在性腺臂中，在那里它们变成内有丝分裂。emo-1编码Sec 61 p γ亚基的同源物，Sec 61 p γ亚基是将分泌和跨膜蛋白易位到酵母和哺乳动物细胞的内质网中所必需的蛋白质。一个假定的emo-1无效突变oz 151显示胚胎致死性。oz 1不育突变是一个转座因子插入emo-1 3'非翻译区，几乎完全消除生殖细胞mRNA的积累。利用oz 1等位基因进行的遗传镶嵌分析表明，生殖细胞中emo-1（+）的表达是生育所必需的。识别卵母细胞表面抗原的J67单克隆抗体（Strome，S. 1986.配子发生和早期胚胎。J.G.加尔，编辑。Alan R.利斯公司，纽约。77-95.），不染色OZ 1卵母细胞，这一发现与突变体中蛋白质转运缺陷一致。我们推测emo-1基因产物在C. elegans卵母细胞，并且是卵子发生和排卵与减数分裂成熟耦合所必需的。
emo-1(oz1) is a member of a class of hermaphrodite sterile mutations in Caenorhabditis elegans that produce endomitotic oocytes in the gonad arm. Oocytes in emo-1(oz1) mutants exhibit multiple defects during oogenesis. After meiotic maturation, ovulation fails, trapping oocytes in the gonad arm where they become endomitotic. emo-1 encodes a homologue of the Sec61p gamma subunit, a protein necessary for translocation of secretory and transmembrane proteins into the endoplasmic reticulum of yeast and mammalian cells. A putative emo-1 null mutation, oz151, displays embryonic lethality. The oz1 sterile mutation is a transposable element insertion into the emo-1 3' untranslated region that almost completely eliminates germline mRNA accumulation. Genetic mosaic analysis using the oz1 allele indicates that emo-1(+) expression in germ cells is required for fertility. The J67 monoclonal antibody, which recognizes an oocyte surface antigen (Strome, S. 1986. In Gametogenesis and the Early Embryo. J.G. Gall, editor. Alan R. Liss, Inc., New York. 77-95.), does not stain oz1 oocytes, a finding consistent with defective protein transport in the mutant. We propose that the emo-1 gene product acts in the transport of secreted and transmembrane proteins in C. elegans oocytes, and is necessary for both oogenesis and the coupling of ovulation with meiotic maturation.