Regulation of nuclear envelope assembly disassembly by MAP kinase
Regulation of nuclear envelope assembly disassembly by MAP kinase
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DOI：
10.1006/dbio.1996.0121
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发表时间：
1996-05-01
影响因子：
2.7
通讯作者：
Kopf, GS
中科院分区：
文献类型：
--
作者：
Moos, J;Xu, Z;Kopf, GS
在中期 II 中被捕的小鼠卵表现出高水平的 cdc2/cyclin B1 和 MAP 蛋白激酶活性。受精后，每种蛋白激酶的活性都会随时间而下降。 cdc2/cyclin B1 蛋白激酶的下降与减数分裂的恢复和第二极体的排出相关，并且先于 MAP 激酶活性的下降，而 MAP 激酶活性在时间上与雄性和雌性原核被膜的形成相关。这些结果表明高水平的 MAP 激酶活性与原核被膜的存在不相容。为了测试这种可能性，我们在小鼠卵中表达了 MAP 激酶激酶 (MEK) 的组成型活性形式，其唯一已知的靶标是 p42/p44 MAP 激酶。我们发现受精后 cdc2/cyclin B1 激酶活性下降，并释放出第二个极体。然而，内源性 MAP 激酶仍然活跃，并且没有原核包膜形成。因此，小鼠卵中高水平的 MAP 激酶活性本身似乎与原核被膜的存在不相容。 (C) 1996 学术出版社
Mouse eggs arrested in metaphase II display high levels of cdc2/cyclin B1 and MAP protein kinase activities. Following fertilization there is a time-dependent decrease in the activity of each of these protein kinases. The decline in cdc2/cyclin B1 protein kinase correlates with the resumption of meiosis and the emission of the second polar body and precedes the decline in MAP kinase activity, which correlates temporally with the formation of the male and female pronuclear envelopes. These results suggest that high levels of MAP kinase activity are incompatible with the presence of a pronuclear envelope. To test this possibility, we expressed in mouse eggs a constitutively active form of MAP kinase kinase (MEK) whose only known target is p42/p44 MAP kinase. We show that following fertilization cdc2/cyclin B1 kinase activity declines and a second polar body is emitted. The endogenous MAP kinase remains active, however, and no pronuclear envelopes form. Thus, high levels of MAP kinase activity by itself in mouse eggs appear incompatible with the presence of a pronuclear envelope. (C) 1996 Academic Press, Inc.