Hypertension affects the biocompatibility and biomineralization of MTA, High-plasticity MTA, and Biodentine®
Hypertension affects the biocompatibility and biomineralization of MTA, High-plasticity MTA, and Biodentine®
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DOI：
10.1590/1807-3107bor-2019.vol33.0060
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发表时间：
2019-01-01
影响因子：
2.5
通讯作者：
GOMES FILHO, João Eduardo
中科院分区：
文献类型：
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作者：
COSME-SILVA, Leopoldo;DAL-FABBRO, Renan;GOMES FILHO, João Eduardo
本研究评估了高血压对大鼠组织反应和白色三氧化二矿物质聚集体 (MTA)、高可塑性 MTA (MTA HP) 和 Biodentine (R) (BDT) 生物矿化能力的影响。将装有 MTA、MTA HP、BDT 和对照组（空管）的聚乙烯管放入 32 只雄性大鼠（16 只正常血压 (NT) 和 16 只高血压大鼠 - 每组 8 只）的背部皮下组织中。 7天和30天后，将结缔组织包围的聚乙烯管取出、固定并嵌入组织学树脂中。在HE染色切片中估计炎症细胞的平均数量，通过Kossa (VK)染色将生物矿化量化为面积(μ m(2))，并通过偏振光(LP)显微镜进行检查。根据 Shapiro-Wilk 正态性检验，通过 Mann-Whitney 或 Student t 检验对各组之间的差异进行统计分析 (p < 0.05)。高血压大鼠对所有材料的炎症反应均高于 NT 大鼠 (p < 0.05)。 NT 大鼠中 MTA 和 BDT 中的阳性 VK 染色在第 7 天和第 30 天时更为明显 (p < 0.05)。 7 天时 NT 大鼠中，MTA、MTA HP 和 BDT 的 LP 中的双折射结构更为明显（p
This study evaluated the effect of hypertension on tissue response and biomineralization capacity of white Mineral Trioxide Aggregate (MTA), High-plasticity MTA (MTA HP), and Biodentine (R) (BDT) in rats. Polyethylene tubes filled with MTA, MTA HP, BDT, and the control group (empty tubes) were placed into the dorsal subcutaneous tissue of 32 male rats (16 normotensive (NT) and 16 hypertensive rats - 8 per group). After 7 and 30 days, the polyethylene tubes surrounded by connective tissue were removed, fixed, and embedded in histological resin. The mean number of inflammatory cells was estimated in HE-stained sections, biomineralization was quantified as area (mu m(2)) by Kossa (VK) staining, and examination by polarized light (LP) microscopy was performed. The differences amongst the groups were analyzed statistically by the Mann-Whitney or Student's t test, according to Shapiro-Wilk test of normality (p < 0.05). The inflammatory responses to all materials were greater in hypertensive rats than in NT rats (p < 0.05). Positive VK staining in MTA and BDT were more pronounced in NT rats at 7 and 30 days (p < 0.05). Birefringent structures in LP for MTA, MTA HP, and BDT were more pronounced in NT rats at 7 days (p