Using GFP in FRET-based applications
Using GFP in FRET-based applications
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DOI：
10.1016/s0962-8924(98)01434-2
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发表时间：
1999-02-01
影响因子：
19
通讯作者：
Heim, R
中科院分区：
文献类型：
--
作者：
Pollok, BA;Heim, R
使用绿色荧光蛋白（GFP）是一种强大的技术，最近使研究人员能够研究活细胞内的动态分子事件。用于检测分子相互作用的一种方法涉及两个GFP之间或GFP与第二荧光团之间的荧光共振能量转移（FRET）。这篇综述总结了使用绿色荧光蛋白的FRET和说明的主题与具体的例子，如何绿色荧光蛋白已被用作细胞内的分子传感器。
The use of green fluorescent protein (GFP) is a powerful technology that has recently enabled investigators to study dynamic molecular events within living cells. One method for detecting molecular interactions involves fluorescence resonance energy transfer (FRET) between two GFPs or between GFP and a second fluorophore. This review summarizes the use of GFP for FRET and illustrates the theme with specific examples on how GFP has been employed as an intracellular molecular sensor.