Inhibition of gold nanoparticles (AuNPs) on pathogenic biofilm formation and invasion to host cells.
Inhibition of gold nanoparticles (AuNPs) on pathogenic biofilm formation and invasion to host cells.
复制标题
金纳米颗粒 (AuNP) 对致病性生物膜形成和宿主细胞侵袭的抑制作用
DOI：
10.1038/srep26667
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发表时间：
2016-05-25
影响因子：
4.6
通讯作者：
Li M
中科院分区：
文献类型：
--
作者：
Yu Q;Li J;Zhang Y;Wang Y;Liu L;Li M
由于由真核和原核病原体引起的传染病日益增多，迫切需要开发新的抗菌药物来治疗临床病原性感染。生物膜的形成和侵入宿主细胞是病原菌定植和感染过程中的重要过程。在这项研究中，我们测试了金纳米颗粒(AuNPs)对病原体生长、生物被膜形成和侵袭的抑制作用。有趣的是，虽然合成的AuNPs对受试病原菌白色念珠菌和铜绿假单胞菌没有明显的毒性，但它们强烈地抑制了致病生物膜的形成和对牙髓干细胞的侵袭。进一步的研究表明，AuNPs大量结合到病原体细胞上，这可能是它们抑制生物被膜形成和侵袭的原因之一。此外，AuNPs治疗导致DPSCs免疫应答相关基因的激活，这可能增强宿主免疫系统对病原体的活性。Zeta电位分析和聚乙二醇/聚乙烯亚胺(PEI)涂层测试进一步表明，病原体细胞与AuNPs之间的相互作用与静电吸引有关。我们的发现为纳米材料在对抗临床病原菌方面的应用提供了新的线索，并暗示在抗菌药物的筛选中，传统的生长抑制试验并不是唯一评价药物效果的方法。
Owing to the growing infectious diseases caused by eukaryotic and prokaryotic pathogens, it is urgent to develop novel antimicrobial agents against clinical pathogenic infections. Biofilm formation and invasion into the host cells are vital processes during pathogenic colonization and infection. In this study, we tested the inhibitory effect of Au nanoparticles (AuNPs) on pathogenic growth, biofilm formation and invasion. Interestingly, although the synthesized AuNPs had no significant toxicity to the tested pathogens,Candida albicansandPseudomonas aeruginosa, the nanoparticles strongly inhibited pathogenic biofilm formation and invasion to dental pulp stem cells (DPSCs). Further investigations revealed that AuNPs abundantly bound to the pathogen cells, which likely contributed to their inhibitory effect on biofilm formation and invasion. Moreover, treatment of AuNPs led to activation of immune response-related genes in DPSCs, which may enhance the activity of host immune system against the pathogens. Zeta potential analysis and polyethylene glycol (PEG)/polyethyleneimine (PEI) coating tests further showed that the interaction between pathogen cells and AuNPs is associated with electrostatic attractions. Our findings shed novel light on the application of nanomaterials in fighting against clinical pathogens, and imply that the traditional growth inhibition test is not the only way to evaluate the drug effect during the screening of antimicrobial agents.