A Transcriptional Network Promotes Anthocyanin Biosynthesis in Tomato Flesh.
A Transcriptional Network Promotes Anthocyanin Biosynthesis in Tomato Flesh.
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DOI：
10.1016/j.molp.2019.10.010
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发表时间：
2020-01
期刊：
影响因子：
27.5
通讯作者：
Chuanlong Sun;Lei Deng;Minmin Du;Jiuhai Zhao;Qian Chen;Tingting Huang;Hongling Jiang;Changbao Li;Chuanyou Li
中科院分区：
文献类型：
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作者：
Chuanlong Sun;Lei Deng;Minmin Du;Jiuhai Zhao;Qian Chen;Tingting Huang;Hongling Jiang;Changbao Li;Chuanyou Li
膳食花青素是重要的促进健康的抗氧化剂，对水果的质量做出了重大贡献。有趣的是，大多数番茄品种在果实中不产生花青素。然而，紫色番茄品种靛蓝玫瑰（Indigo Rose）具有野生番茄物种的显性Aft基因座和隐性atv基因座结合，在果皮中表现出光依赖性花色苷积累。在这里，我们报告说Aften编码一种名为SlAN 2-like的功能性花青素激活剂，而Aften编码一种非功能性花青素抑制剂SlMYBATV。SlAN 2-like基因的表达对光照有响应性，并且具有功能性的SlAN 2-like基因能够激活花色素苷生物合成基因及其调控基因的表达，表明SlAN 2-like基因在花色素苷生物合成的激活过程中起着主调节因子的作用。我们进一步表明，栽培番茄含有SlAN 2样的非功能等位基因，因此不能产生花青素。因此，在番茄品种中由果实特异性启动子驱动的功能性SlAN 2-like基因的表达导致了整个花青素合成途径的激活，并在果皮和果肉中高水平积累花青素。总之，我们的研究证实，复杂的代谢途径的有效工程可以通过主转录调节因子的组织特异性表达来实现。
Dietary anthocyanins are important health-promoting antioxidants that make a major contribution to the quality of fruits. It is intriguing that most tomato cultivars do not produce anthocyanins in fruit. However, the purple tomato variety Indigo Rose, which has the dominantAftlocus combined with the recessiveatvlocus from wild tomato species, exhibits light-dependent anthocyanin accumulation in the fruit skin. Here, we report thatAftencodes a functional anthocyanin activator named SlAN2-like, whileatvencodes a nonfunctional version of the anthocyanin repressor SlMYBATV. The expression ofSlAN2-likeis responsive to light, and the functional SlAN2-like can activate the expression of both anthocyanin biosynthetic genes and their regulatory genes, suggesting that SlAN2-like acts as a master regulator in the activation of anthocyanin biosynthesis. We further showed that cultivated tomatoes contain nonfunctional alleles ofSlAN2-likeand therefore fail to produce anthocyanins. Consistently, expression of a functionalSlAN2-likegene driven by the fruit-specific promoter in a tomato cultivar led to the activation of the entire anthocyanin biosynthesis pathway and high-level accumulation of anthocyanins in both the peel and flesh. Taken together, our study exemplifies that efficient engineering of complex metabolic pathways could be achieved through tissue-specific expression of master transcriptional regulators.