Combined effect of microplastics and DDT on microbial growth: A bacteriological and metabolomics investigation in Escherichia coli
Combined effect of microplastics and DDT on microbial growth: A bacteriological and metabolomics investigation in Escherichia coli
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微塑料和滴滴涕对微生物生长的综合影响：大肠杆菌的细菌学和代谢组学研究
DOI：
10.1016/j.jhazmat.2020.124849
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发表时间：
2021-04-05
影响因子：
13.6
通讯作者：
Ouyang, Gangfeng
中科院分区：
文献类型：
--
作者：
Liu, Shuqin;Fang, Shuting;Ouyang, Gangfeng
微塑料(MPS)可以吸附生物或环境基质中的有毒化学物质，从而影响它们的行为和可用性。为了研究MPS和有毒有机物的复合污染如何影响微生物的生长和代谢，在一个复杂的、明确的介质中培养了大肠杆菌，并在人体相应的浓度水平上用聚苯乙烯微塑料(PS MPS)和二氯二苯基三乙烷(DDT)进行了处理。利用一种新型固相微萃取(SPME)探针捕获的体内代谢产物用于反映不同污染胁迫下大肠杆菌的代谢失调情况。结果表明，DDT的毒性效应表现出明显的剂量依赖性，PS的存在降低了DDT对大肠杆菌的生长和代谢干扰效应。吸附结果揭示了PS通过降低培养液中DDT的游离浓度来减弱DDT的不利影响的机制。大肠杆菌中的三羧酸(TCA)循环相关酶活性和抗氧化防御相关物质也证实了这一机制。本研究有助于加深我们对MPS与有毒有机物对微生物的生态毒性的认识。
Microplastics (MPs) can adsorb toxic chemicals in biological or environmental matrixes and thus influence their behavior and availability. In order to investigate how the combined pollution of MPs and toxic organic chemical influence microbial growth and metabolism, Escherichia coli (E. coli) was grown in a complex, well-defined media and treated with polystyrene microplastics (PS MPs) and dichloro-diphenyl-tricgloroethane (DDT) at human relevant concentration levels. In vivo metabolites captured by a novel solid phase microextraction (SPME) probe, were used to reflect the metabolic dysregulation of E. coli under different pollution stresses. Results showed that the toxic effect of DDT displayed a distinct dose-dependent phenomenon while the existence of PS decreased the growth and metabolic interference effect of DDT on E. coli. Adsorption results revealed a mechanism that PS weakened the adverse impact of DDT by decreasing its free concentration in the treated culture media. Tricarboxylic acid (TCA) cycle related enzymes activities and antioxidant defense related substances of E. coli also proved the mechanism. The current study is believed to broaden our understanding of the ecotoxicity of MPs with toxic organic chemicals on microorganism.