Effects of prostaglandin E1 on collagen production and degradation in human fetal lung fibroblasts.
Effects of prostaglandin E1 on collagen production and degradation in human fetal lung fibroblasts.
复制标题
前列腺素 E1 对人胎肺成纤维细胞胶原蛋白生成和降解的影响。
DOI：
10.1016/0003-9861(88)90147-6
复制
发表时间：
1988
影响因子：
3.9
通讯作者：
Bienkowski,RS
中科院分区：
文献类型：
--
作者：
Barile,FA;Ripley-Rouzier,C;Siddiqi,ZE;Bienkowski,RS
我们观察了前列腺素E_1对人胎肺成纤维细胞胶原合成和降解的影响。将融合培养物与[~3H]Pro、[14C]甘氨酸或[14C]亮氨酸孵育6h，并测量胶原酶敏感材料和胶原酶不敏感材料中放射性的相对量，以此来测定胶原产量百分比。胶原降解率是通过测量低分子量组分中的羟基[14C]脯氨酸相对于总羟基[14C]脯氨酸来确定的。当前列腺素E_1的浓度低至0.25μg/ml时，胶原蛋白的净产量减少一半，从8±2%降至4±1%(P<；0.05)。相反，降解率的变化相对平缓，从对照组的15±2%稳定上升到4μg/ml时的33±2%(P<；0.05)。降解率的增加虽然显著，但不能解释胶原蛋白产量的总体下降。我们得出结论，前列腺素E1通过两种基本独立的方式抑制胶原的产生：降低合成速度和增加细胞内降解。然而，合成的减少大于降解的增加。
We examined the effects of prostaglandin E1 on the production and degradation of collagen in human fetal lung fibroblasts. Percentage collagen production was determined by incubating confluent cultures for 6 h with [3H]proline and either [14C]glycine or [14C]leucine and measuring the relative amounts of radioactivity incorporated into collagenase-sensitive and collagenase-insensitive material. Percentage collagen degradation was determined by measuring hydroxy[14C]proline in a low-molecular-weight fraction relative to total hydroxy[14C]proline. Prostaglandin E1, when present at a concentration as low as 0.25 μg/ml, reduced net collagen production by a factor of one-half, from 8 ± 2 to 4 ± 1 % (P< 0.05). In contrast, the change in percentage degradation was relatively gradual, rising steadily from the control value of 15 ± 2 to 33 ± 2% at 4 μg/ml (P< 0.05). The increase in degradation, while significant, could not account for the total decrease in collagen production. We conclude that prostaglandin E1 exerts its inhibitory effect on collagen production in two essentially independent ways: lowering the rate of synthesis and increasing intracellular degradation. However, the decrease in synthesis is greater than the increase in degradation.