Anti-Inflammatory Response and Muscarinic Cholinergic Regulation during the Laxative Effect of Asparagus cochinchinensis in Loperamide-Induced Constipation of SD Rats
Anti-Inflammatory Response and Muscarinic Cholinergic Regulation during the Laxative Effect of Asparagus cochinchinensis in Loperamide-Induced Constipation of SD Rats
复制标题
DOI：
10.3390/ijms20040946
复制
发表时间：
2019-02-02
影响因子：
5.6
通讯作者：
Hwang, Dae Youn
中科院分区：
文献类型：
--
作者：
Kim, Ji Eun;Park, Ji Won;Hwang, Dae Youn
据报道，几种类型的皂苷和含有皂苷的草本植物具有抗炎或通便活性。为验证天冬总皂苷提取物(SPA)的抗炎作用和对胃肠系统胆碱能调节的作用，观察了SPA对洛哌丁胺(Lop)诱导的大鼠便秘模型的便秘表型、炎症反应和M胆碱能调节的影响。Lop+SPA治疗组大鼠的大便总数、胃肠通过时间、粘膜层厚度、肠腔表面平坦度、Paneth细胞数和脂滴数均明显高于Lop+赋形剂治疗组。SPA治疗后炎症细胞因子(TNF-、IL-1和IL-6)、炎症介质(核因子-B和诱导型一氧化氮合酶)、肥大细胞总数和粘蛋白分泌恢复。此外，在乙酰胆碱酯酶(AChE)活性和肌球蛋白轻链(MLC)的磷酸化水平以及M受体M2/M3(mAChR M2/M3)及其介体的表达方面也有类似的改善。本研究首次为SPA在LOP诱导的慢性便秘模型中发挥通便作用时能刺激抗炎反应和M胆碱能调节作用提供了强有力的证据。
Several types of saponins and herbal plants containing saponins have been reported to have anti-inflammatory or laxative activities. To verify the therapeutic effects of saponin-enriched extracts of Asparagus cochinchinensis (SPA) on the anti-inflammatory response and on the cholinergic regulation in the gastrointestinal system, an alteration on the constipation phenotypes, on the inflammatory responses, and on the muscarinic cholinergic regulation were investigated in the transverse colons of Sprague Dawley (SD) rats with loperamide (Lop)-induced constipation after the treatment of SPA. Significant increases were observed on the total number of stools, the gastrointestinal transit, the thickness of the mucosal layer, the flat luminal surface, the number of paneth cells, and the lipid droplets in the Lop + SPA-treated group as compared to the Lop + Vehicle-treated group. SPA treatment induced the recovery of inflammatory cytokines (TNF-, IL-1) and IL-6), inflammatory mediators (NF-B and iNOS), the total number of infiltered mast cells, and mucin secretion. Also, some similar improvements were observed on the levels of acetylcholine esterase (AChE) activity and on the phosphorylation of myosin light chains (MLC) as well as the expression of muscarinic acetylcholine receptors M2/M3 (mAChR M2/M3) and their mediators. The results presented herein provide the first strong evidence that SPA stimulates anti-inflammatory responses and the muscarinic cholinergic regulation when exerting its laxative effects in the chronic constipation of Lop-induced models.