Evaluation of Electron Microscopic Sample Preparation Methods and Imaging Techniques for Characterization of Cell-Mineral Aggregates
Evaluation of Electron Microscopic Sample Preparation Methods and Imaging Techniques for Characterization of Cell-Mineral Aggregates
复制标题
DOI：
10.1080/01490450802153462
复制
发表时间：
2008-06
影响因子：
2.3
通讯作者：
Sebastian Schädler;Claus Burkhardt;Andreas Kappler
中科院分区：
文献类型：
--
作者：
Sebastian Schädler;Claus Burkhardt;Andreas Kappler
扫描电子显微镜（SEM）用于对地质微生物样品进行成像，这些样品通常包含矿物和细胞等“硬材料和软材料”之间的界面，这对高分辨率成像的无伪影制备提出了挑战。我们使用微生物Fe（II）氧化和Fe（III）还原过程中产生的细胞矿物聚集体来评估不同的样品制备和成像技术。快速冷冻和标准临界点干燥（CPD）保存的地质微生物样品的结构，至少获得的Fe（II）-氧化和Fe（III）-还原细菌，没有文物。我们推荐了一种用于地质微生物标本的SEM样品制备方案，并讨论了固定、脱水、涂层和加速电压等关键参数。
Scanning Electron Microscopy (SEM) is used to image geomicrobiological samples, typically containing interfaces between “hard and soft materials” such as minerals and cells, which represent challenges for artifact-free preparation for high-resolution imaging. We used cell-mineral aggregates produced during microbial Fe(II) oxidation and Fe(III) reduction to evaluate different sample preparation and imaging techniques. Both rapid freezing and standard critical point drying (CPD) preserve structures of geomicrobiological samples, at least the ones obtained for Fe(II)-oxidizing and Fe(III)-reducing bacteria, without artifacts. We recommend a SEM sample preparation scheme for geomicrobiological specimens and discuss critical parameters like fixation, dehydration, coating, and acceleration voltages.