Dioxygen diffusion in the stratum corneum: an EPR spin label study.
Dioxygen diffusion in the stratum corneum: an EPR spin label study.
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角质层中的分子氧扩散：EPR 自旋标记研究。
DOI：
10.1016/0005-2736(93)90026-v
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
Plachy,WZ
中科院分区：
文献类型：
--
作者：
Hatcher,ME;Plachy,WZ
角质层是皮肤的外层10 μm，是调节人体与环境之间分子运输的渗透性屏障。本研究的目的是了解这种渗透屏障功能，因为它与分子氧的扩散有关。用硬脂酸自旋探针对接种后的角质层进行了EPR光谱研究。顺磁性分子氧的存在使自旋探针的EPR谱线展宽。氧在角质层上的扩散速率，以及氧的扩散系数D (o2)，是通过研究这种线展宽作为时间的函数来确定的。在37°C时，人体角质层中的D （o2）为3·10−7 cm 2/s，活化能约为。44焦每摩尔。在角质层上施用增渗剂DeMSO和DMSO可使D （o2）增加2 - 3倍。
The stratum corneum, the outer 10 μm of the skin, serves as a permeability barrier regulating the transport of molecules between the body and the environment. The purpose of this study is to understand this permeability barrier function as it pertains to the diffusion of molecular oxygen. The stratum corneum was investigated with EPR spectroscopy following inoculation with a stearic acid spin probe. The presence of paramagnetic molecular oxygen results in the broadening of the EPR spectral lines of the spin probe. The rate of oxygen diffusion across the stratum corneum, and then the oxygen diffusion coefficient, D (O 2), was determined by studying this line-broadening as a function of time. D (O 2) in human stratum corneum was found to be 3· 10− 7 cm 2/s at 37° C with an activation energy of approx. 44 kJ/mol. The application of the permeation-enhancing chemicals, DeMSO and DMSO, to the stratum corneum increased D (O 2) two-to three-fold.