The mouse Enhancer trap locus 1 (Etl-1): a novel mammalian gene related to Drosophila and yeast transcriptional regulator genes.
The mouse Enhancer trap locus 1 (Etl-1): a novel mammalian gene related to Drosophila and yeast transcriptional regulator genes.
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小鼠增强子陷阱基因座 1 (Etl-1)：一种与果蝇和酵母转录调节基因相关的新型哺乳动物基因。
DOI：
10.1016/0925-4773(92)90030-n
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发表时间：
1992
影响因子：
2.6
通讯作者：
Gossler,A
中科院分区：
文献类型：
--
作者：
Soininen,R;Schoor,M;Henseling,U;Tepe,C;Kisters-Woike,B;Rossant,J;Gossler,A
一种新的小鼠基因，增强子陷阱位点1（Etl-1），被鉴定为与小鼠基因组中的alacZenhancer陷阱整合非常接近，在发育过程中显示出特异性β-半乳糖苷酶染色模式。原位分析显示Etl-1在整个发育过程中广泛但非普遍表达，在中枢神经系统和上皮细胞中表达水平特别高。从cDNA推导的Etl-1蛋白的氨基酸序列显示出很强的相似性，在500个氨基酸的延伸上，涉及同源异型基因调节的果蝇蛋白质和酵母转录激活蛋白SNF 2/SWI 2以及RAD 54蛋白和最近描述的解旋酶相关酵母蛋白STH 1和MOT 1。1是这组蛋白质中第一个参与基因调控和/或影响染色质结构的哺乳动物成员。Etl-1蛋白与调控蛋白SNF 2/SWI 2和brahma的同源性以及在胚胎发育过程中的表达模式表明，Etl-1蛋白可能参与了小鼠发育过程中的基因调控途径。
A novel mouse gene,Enhancer trap locus 1(Etl-1), was identified in close proximity to alacZenhancer trap integration in the mouse genome showing a specific β-galactosidase staining pattern during development. In situ analysis revealed a widespread but not ubiquitous expression ofEtl-1throughout development with particularly high levels in the central nervous system and epithelial cells. The amino acid sequence of theEtl-1protein deduced from the cDNA shows strong similarity, over a stretch of 500 amino acids, to theDrosophilabrahma protein involved in the regulation of homeotic genes and to the yeast transcriptional activator protein SNF2/SWI2 as well as to the RAD54 protein and the recently described helicase-related yeast proteins STH1 and MOT1.Etl-1is the first mammalian member of this group of proteins that are implicated in gene regulation and/or influencing chromatin structure. The homology to the regulatory proteins SNF2/SWI2 and brahma and the expression pattern during embryogenesis suggest thatEtl-1protein might be involved in gene regulating pathways during mouse development.