Mammalian sperm capacitation and fertilization in vitro: a critique of methodology
Mammalian sperm capacitation and fertilization in vitro: a critique of methodology
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哺乳动物精子获能和体外受精：对方法论的批判
DOI：
10.1002/mrd.1120010210
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发表时间：
1978
期刊：
影响因子：
--
通讯作者：
B. Rogers
中科院分区：
文献类型：
--
作者：
B. Rogers
获能是发生在哺乳动物精子射精后和获得卵子受精能力之前的一个过程。这一过程于1951年首次被认识，在此期间，发现者Austin和Chang以及其他人撰写了大量评论[Austin，1967，1968，1970; Cliang 1959; Brackett，1970，197 1;贝德福德，1970，1972; Srivastava和威廉姆斯，1970;威廉姆斯，1972; McRorie和威廉姆斯，1974年;巴罗斯，1974年; Zaneveld，1975年; Gwatkin，19761年。体外获能和受精领域一直受到阻碍，因为新的研究人员无法重现一些经典的实验，这些实验已经成为教条的一部分。本综述是对最新技术水平的评价，重点是方法学和重现性。自1970年贝德福德的优秀评论以来，出现了大量的新发展和评论。当时，仅在仓鼠（1963年）、小鼠（1969年）、人（1969年）和中国仓鼠（1969年）中成功实现了单一系统的体外获能和受精。目前已在其他7个物种中证明了这一点;豚鼠（1972）、蒙古沙鼠（1973）、猴（1973）、大鼠（1974）、兔（1975）、犬（1976）和猫（1977）。每一个物种，其中获能和体外受精已经实现将进行审查。所包括的论文并不包括使用特定的体外系统所做的工作，但已被选择来展示方法学的进步，并提供体外获能和受精的重要参数的可能的澄清。
Capacitation is a process that occurs in mammalian spermatozoa after they have been ejaculated and before they acquire the ability to fertilize eggs. This process was first recognized in 1951 and numerous reviews have been written in the intervening years by Austin and Chang, the discoverers, and by others [Austin, 1967, 1968, 1970; Cliang 1959; Brackett, 1970, 197 1; Bedford, 1970, 1972; Srivastava and Williams, 1970; Williams, 1972; McRorie and Williams, 1974; Barros, 1974; Zaneveld, 1975; Gwatkin, 19761. The field of capacitation and fertilization in vitro has been hampered by the inability of new investigators to reproduce some of the classical experiments which have become part of the dogma. This review is an evaluation of the state of the art with an emphasis on methodology and reproducibility. A plethora of new developments and reviews have appeared since Bedford’s excellent review in 1970. At that time, successful in vitro capacitation and fertilization in a single system had been achieved only in hamster (1963), mouse (1969), human (1969), and Chinese hamster (1969). At present this has been demonstrated in seven other species; guinea pig (1972), Mongolian gerbil (1973), monkey (1973), rat (1974), rabbit (1975), dog (1976), and cat (1977). Each species in which capacitation and fertilization in vitro has been achieved will be reviewed. Papers included are not all inclusive of work done using a particular in vitro system but have been selected to demonstrate advances in methodology and provide possible clarification of the important parameters of capacitation and fertilization in vitro.