A core functional region of the RFP1 promoter from Chinese wild grapevine is activated by powdery mildew pathogen and heat stress
A core functional region of the RFP1 promoter from Chinese wild grapevine is activated by powdery mildew pathogen and heat stress
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DOI：
10.1007/s00425-012-1769-9
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发表时间：
2012-09
期刊：
影响因子：
4.3
通讯作者：
Yihe Yu;Weirong Xu;Jie Wang;Lei Wang;Wenkong Yao;Yan Xu;Jiahua Ding;Yuejin Wang
中科院分区：
文献类型：
--
作者：
Yihe Yu;Weirong Xu;Jie Wang;Lei Wang;Wenkong Yao;Yan Xu;Jiahua Ding;Yuejin Wang
环指蛋白（RFP）是一类泛素连接酶，在植物对生物和非生物胁迫的应答中起着重要作用。然而，关于RFP表达调控的信息很少。本研究从抗病的中国野生葡萄葡萄品种百合-35-1中分离并鉴定了RFP启动子序列。启动子-GUS融合分析表明，防御信号分子，白粉病感染，热胁迫诱导VpRFP 1启动子活性。与此相反，从葡萄中分离的RFP 1启动子仅受到病原菌感染和热处理的轻微诱导。通过启动子缺失分析，我们发现VpRFP 1启动子的−148 bp区域是核心功能启动子区域。我们还发现，在拟南芥中，VpRFP 1在其自身启动子下的表达激活了防御相关基因的表达，提高了抗病性，但使用VvRFP 1启动子的同一构建体对抗病性的提高幅度不大。我们的研究结果表明VpRFP 1启动子的−148 bp区域在响应病原菌和热胁迫中起着关键作用，并表明VpRFP 1和VvRFP 1之间的表达差异可能是两种葡萄基因型不同抗病表型的关键。
RING-finger proteins (RFP) function as ubiquitin ligases and play key roles in plant responses to biotic and abiotic stresses. However, little information is available on the regulation ofRFPexpression. Here, we isolate and characterize theRFPpromoter sequence from the disease-resistant Chinese wild grapeVitis pseudoreticulataaccession Baihe-35-1. Promoter-GUS fusion assays revealed that defense signaling molecules, powdery mildew infection, and heat stress induceVpRFP1promoter activity. By contrast, theRFP1promoter isolated fromVitis viniferawas only slightly induced by pathogen infection and heat treatment. By promoter deletion analysis, we found that the −148 bp region of theVpRFP1promoter was the core functional promoter region. We also found that, inArabidopsis,VpRFP1expressed under its own promoter activated defense-related gene expression and improved disease resistance, but the same construct using theVvRFP1promoter slightly improve disease resistance. Our results demonstrated that the −148 bp region of theVpRFP1promoter plays a key role in response to pathogen and heat stress, and suggested that expression differences betweenVpRFP1andVvRFP1may be key for the differing disease resistance phenotypes of the twoVitisgenotypes.