RAPID DERIVATIZATION AND GAS-CHROMATOGRAPHIC DETERMINATION OF AMINO-ACIDS
RAPID DERIVATIZATION AND GAS-CHROMATOGRAPHIC DETERMINATION OF AMINO-ACIDS
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DOI：
10.1016/s0021-9673(01)95945-x
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发表时间：
1991-08-09
期刊：
影响因子：
--
通讯作者：
HUSEK, P
中科院分区：
文献类型：
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作者：
HUSEK, P
建立了氯甲酸酯衍生化氨基酸的反应条件。在水性反应介质中，所述试剂不仅能够使侧链反应性基团水解，而且甚至能够使羧基水解。用毛细管气相色谱法测定氨基酸的合适衍生物是N（O，S）-乙氧羰基乙酯，它在几秒钟内形成。用衍生化蛋白质氨基酸的混合物测试了六种固定相。OV-1701型中等极性固定相可在5分钟内以良好的分离度测定氨基酸。
Reaction conditions for the derivatization of amino acids with chloroformates were established. In an aqueous reaction medium the reagents are able to esterify not only the side-chain reactive groups but even the carboxylic group. Suitable derivatives for the determination of amino acids by capillary gas chromatography were found to be the N(O,S)-ethoxycarbonyl ethyl esters, which are formed in a few seconds. Six stationary phases were tested with a mixture of derivatized protein amino acids. A moderately polar stationary phase of the OV-1701 type permits the determination of amino acids with good resolution in less than 5 min.